微卫星DNA vWA的多态性与精神分裂症的关联研究

目的　通过对微卫星DNAvWA多态性的分析 ,了解精神分裂症与vWA有关“等位基因”的关联情况。方法　对 32例精神分裂症患者和 12 3名随机人群采用PEProfilerplus系统进行PCR复合扩增 ,然后用ABI310型基因分析系统对扩增产物进行电泳和基因检测。结果　两组vWA“等位基因”频率符合Hardy Weinberg平衡定律 (P >0 0 5 ) ;精神分裂症组vWA 14的检出率为 17 2 % ,对照组的检出率为 33 3% ,两组有显著性差异 (P <0 0 5 ) ;精神分裂症组vWA 17的检出率为 31 2 % ,对照组检出率为 19 5 % ,两组有显著性差异 (P <0 0 5 ) ;其他各“等位基因”检出率两组无统计学显著差异 (P >0 0 5 )。结论　vWA“等位基因”vWA 14和vWA 17与精神分裂症的发病有关 ,在第 12号染色体上可能存在与精神分裂症相关的基因

Association between vWA gene polymorphism and schizophrenia in the Chinese population

Objective The aim of this paper was to explore association between vWA gene polymorphism and schizophrenia. Methods DNA samples were collected from patients with schizophrenia (n=32) and random population (n=123) in China. The vWA, a short tandem repeats gene, was determined using the STR Profiler Plus PCR amplification kit. Results No deviation from the Hardy-Weinberg equilibrium was observed. Frequency of vWA-14 was lower in schizophrenia group (f=17.2%) than in control group (f=33.3%) (P<0.05). Frequency of vWA-17 was 31.2% in schizophrenia group and 19.5% in control group and difference was significant(P<0.05). There was not statistical difference in allele frequencies between two groups. Conclusions vWA alleles may be associated with schizophreniz. It is suggested that there are sensitive or resistance genes in chromosome 12.