胃癌细胞系中端粒酶活性相关基因的表达

目的 检测胃癌细胞系中端粒酶RNA干涉后是否改变其他基因的表达。方法 根据RNA干涉原理，针对端粒酶基因序列设计了抑制端粒酶表达的核苷酸序列，克隆入真核表达载体中；并以脂质体转入到胃癌细胞系的MGC80-3和BGC-823细胞中，以空质粒为对照．48h后收集细胞的总RNA，逆转录成eDNA后．通过荧光差异显示法检测基因表达的变化。结果 胃癌细胞BGE-823细胞和MGC-0．3细胞中一些基因的表达量明显下降。结论 荧光差异显示法能比较系统地检测与端粒酶活性相关的基因，为进一步研究这些基因与端粒酶之间的表达调控方式及其对细胞生长的影响打下了基础。

Detection of the Genes Related to Telomerase Activity in Stomach Tumor Cell Lines

Objective To detect the alteration of genes expression after RNA interference of Telomerase. Methods According to the principle of RNA interference, a nucleotide sequence was designed to inhibit telomerase gene expression, the sequence was cloned into a eukaryotic vector and transferred into the stomach tumor cell lines, MGCS0-3 and BGC-823 cells, the primary vector as a control, after 48 hours, the total RNA of each test was extracted and was synthesized to cDNA and the alteration of genes expression was detected by fluorescence differential display. Results The reduction of genes expression in MGCS0-3 and BGC-823 cells was observed after the telomerase activity was inhibited. Conclusion The methods are effective in detection of the changes of a series of genes expression after telomerase RNA interference, it will be helpful to understand the relationships between genes and telomerase, and effect on to cell growth.