| 恶性疟原虫重组质粒pVXORF1-PfMSP2-HBS在小鼠骨骼肌的表达 |
| |
| 引用本文： | 赖秀球.恶性疟原虫重组质粒pVXORF1-PfMSP2-HBS在小鼠骨骼肌的表达[J].中国热带医学,2005,5(5):935-936. |
| |
| 作者姓名： | 赖秀球 |
| |
| 作者单位： | 广东医学院寄生虫学教研室,广东,湛江,524023 |
| |
| 摘 要： | 目的 观察恶性疟原虫海南分离株(FCC1／HN)裂殖子表面蛋白2(MSP2)融合HBsAg基因片断真核表达质粒pVXORF1-PiMSP2-HBS在小鼠骨骼肌的表达。方法KM小鼠分3组，用0．5％盐酸布比卡因对3组小鼠行预处理后，在同一部位Ⅰ组注射浓度为1μg／μl的重组质粒pVXORF1-PiMSP2-HBS100μl，Ⅱ组注射浓度为1μg／μ的质粒pVXORFI1-HBS100μl，Ⅲ组为正常对照组，注射pH7．4PBS100μl。分别在免疫后4周和8周用免疫组织化学实验检测小鼠注射部位肌肉组织中重组蛋白MSP2的表达。结果小鼠经DNA疫苗注免疫4周和8周后肌肉组织呈特异性阳性反应。结论重组质粒pVXORF1-PfMSP2-HBS免疫小鼠后，肌肉组织有重组蛋白MSP2的表达。
|
| 关 键 词： | 恶性疟原虫 重组质粒 pVXORF1-PfMSP2-HBS 小鼠 骨骼肌 基因表达 免疫组织化学 |
| 文章编号： | 1009-9727(2005)05-935-02 |
| 修稿时间： | 2005年5月23日 |
The expression of recombinant palsmid pVXORF1-PfMSP2-HBS in the skeletal muscle of mice. |
| |
| LAI Xiu-qiu.The expression of recombinant palsmid pVXORF1-PfMSP2-HBS in the skeletal muscle of mice.[J].China Tropical Medicine,2005,5(5):935-936. |
| |
| Authors: | LAI Xiu-qiu |
| |
| Abstract: | Objective To explore the recombinant protein expression status in muscle tissue of mice vaccinated with plasmid pVXORFl-PfMSP_2-HBS of Plasrnodium falciparurn FCCl/HN strain. Methods The recombinant plasmid pVXORFl-PfMSP_2-HBS, pVXORFl-HBS and pH7.4 PBS were injected into 3 groups of KM mice respectively by intramuscular route, and the expression product of DNA vaccine was detected with immunohistochemistry assay 4 weeks and 8 weeeks after immunization. Results The specific positive reaction of the immunohistochemistry assay were observed in muscle tissue in immune group when vaccinated with plasmid pVXORFl-PfMSP_2-HBS,while regatiue reaetion was obserred in the mice of other two groups. Conclusion After vaccinating KM mice with plasmid pVXORFl-PfMSP_2-HBS by intramuscular injection directly, the recombinant protein MSP2 could be expressed in muscle tissue of mice. |
| |
| Keywords: | Plasmodium falciparurn MSP_2 Immunohistochemistry Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
