高血压患者颈动脉粥样硬化与血清中sICAM-1水平及K469E基因多态性的相关性研究

目的：探讨细胞间黏附分子-1（ICAM-1）K469E基因多态性与高血压患者颈动脉粥样硬化关系及血清中的ICAM-1（sICAM-1）对其影响。方法：采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术以及酶联免疫吸附试验（ELISA）对201例高血压患者和106例对照者的细胞间黏附分子K469E基因多态性及血清中sICAM-1水平检测。结果：高血压组ICAM-1基因型及等位基因频率与对照组比较差异无统计学意义,但在高血压患者中KK基因型颈动脉内膜-中膜厚度（IMT）比E基因携带者高血压患者的厚。高血压患者血清中sICAM-1水平及IMT较对照组增加,并且血清中sICAM-1水平随着高血压患者IMT增厚而增加。结论：在高血压人群中,ICAM-1 K469E基因多态性与颈动脉粥样硬化有关,E等位基因可能是颈动脉硬化遗传易感性基因,而且sICAM-1与颈动脉IMT密切相关。

Study on the association of the K496E gene polymorphisms of ICAM and the serum ICAM-1 in hypertension with the carotid atherosclerosis

Objective： To investigate the relation between the K496E gene polymorphisms and essential hypertension（EH） with the carotid atherosclerosis,and study the correlation between the serum level of ICAM-1 and the carotid atherosclerosis.Methods： 201 hypertension patients and 106 healthy people were selected,PCR-RFLP was used to determine their K496E gene polymorphisms,the serum level of ICAM-1 was measured by ELISE.Results： There was no significant difference in the distribution of ICAM-1 genotypes and allelie freguencies between EH group and the health control group,but in the EH group the EH subjects with KK genotype of carotid artery intima-media thickness（IMT） had increased than E allele carriers.The sICAM-1 levels and IMT were significantly higher in hypertensive patients than those of in the control subjects,and the serum levels of ICAM had an increasing trend with the thickness of IMT.Conclusion： ICAM-1 K469E gene polymorphism is associated with carotid artery arteriosclerosis of hypertension,E allele may be a risk factor for carotid artery arteriosclerosis.The serum ICAM-1 levels in the essential hypertension patients obviously increases in the EH group.