淫羊藿和熟地煎剂对糖皮质激素受体下调模型大鼠糖皮质激素受体的影响

目的 观察温阳、滋阴代表中药淫羊藿、熟地水煎剂对糖皮质激素受体（glucocorticoid receptor，GR）下调模型大鼠GR的影响。方法 皮下注射氢化可的松法，建立GR下调模型。72只SD大鼠随机分为正常组、模型组、淫羊藿组、熟地组，每组再分别分为3个不同时间点组（给药第3、7、14天），每组6只大鼠。采用放免法和流式细胞术检测给药后不同时间点（第3、7、14天）各组大鼠血浆皮质酮水平及脾淋巴细胞GR的蛋白表达和结合力的变化。结果 不同时间点模型组血浆皮质酮水平与正常对照组比较差异均无统计学意义（P>0.05），灌胃给药14天后，熟地组血浆皮质酮水平较模型组降低（P＜0.05）。GR蛋白表达和结合力模型组较正常组明显下降(P＜0.01），熟地给药7天和14天组较模型组均明显上调（P＜0.01），淫羊藿给药14天组较模型组也升高（P＜0.01）。结论 淫羊藿、熟地水煎剂均能不同程度改善GR下调模型大鼠GR的蛋白表达和结合力，但其作用时间点不一致。

Effect of Herba Epimedii Brevicornus and Prepared Radix Rehmannia on Glucocorticoid Receptor in Glu-cocorticoid Receptor Down-regulated Rats

OBJECTIVE: To evaluate the effect of Herba Epimedii Brevicornus (HEB) and prepared Radix Rehmannia (RR), the representative Chinese herbs for warming yang and nourishing yin on glucocorticoid receptor (GR) in GR down-regulated rats. METHODS: The GR down-regulated model was established by subcutaneous injection of hydrocortisone. Seventy-two SD male rats were randomly divided into four groups: the normal group; the model group (MG) ; the HEB group, and the RR group. And every group was subdivided to 3 batches depending on the time points (the 3rd, 7th and 14th day after modeling) of observation, with 6 rats in each batch. Changes in blood level of corticosterone (GS), as well as protein expression and binding power of GR in splenic lymphocytes at corresponding time points of the batches were determined by RIA and flow cytometry. RESULTS: No significantly difference was found in blood GS levels of the model group at all the time points, as compared with that of the normal group (P > 0.05), while that in the RR group was significantly lowered at the 14th day, and showed significant difference from that in the model group (P < 0.05). The protein expression and binding power in the model rats were lower than those in normal ones (all P < 0.01), but they were significantly higher on the 7th and 14th day in the RR group (P < 0.01), and on the 14th day in the HEB group higher than those in the model group, respectively (all P < 0.01). CONCLUSION: Both HEB and RR, in dosage-form of water decoction, could up-regulate the protein expression and binding power of GR in GR down-regulated model rats, but different in acting time.