| 人膀胱移行细胞癌各种透明质酸合成酶基因亚型的表达及意义 |
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| 引用本文： | 蔡建良,辛殿祺,吕天敬,何群,那彦群.人膀胱移行细胞癌各种透明质酸合成酶基因亚型的表达及意义[J].临床泌尿外科杂志,2008,23(5):348-351. |
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| 作者姓名： | 蔡建良 辛殿祺 吕天敬 何群 那彦群 |
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| 作者单位： | 1. 北京大学首钢医院泌尿外科,北京,100041
2. 北京大学第一医院泌尿外科,北京大学泌尿外科研究所 |
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| 摘 要： | 目的:研究各种透明质酸合成酶亚型(HASs)mRNA在人膀胱移行细胞癌中的表达及其潜在的临床价值.方法:运用半定量RT-PCR方法,对145例不同恶性度的人膀胱移行细胞癌、22例正常人膀胱黏膜组织标本和4种人BTCC细胞系的HAS亚型mRNA的表达情况进行研究.结果:PCR 23循环后,正常膀胱黏膜组织中7例扩增出微弱的HASl mRNA条带(28%,7/25),其余无任何条带扩增出来;所有膀胱移行细胞癌组织标本和细胞系有一种以上的HAS亚型mRNA被扩增出来.不同膀胱癌组织、细胞系表达的HASs mRNA的种类及表达程度各不相同,但随着肿瘤恶性程度的增高,其表达的各种HASs mRNA的种类趋向相同.每个膀胱癌组织、细胞系均有一种HASs mRNA优势表达,但其类型及优势表达程度互不相同.结论:人膀胱移行细胞癌出现了HAS亚型mRNA的异常高表达,可能与肿瘤的发生、发展有密切关系,是BTCC生物治疗潜在的靶点;同时进一步证实了尿透明质酸作为膀胱癌的"标志物"的可靠性.
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| 关 键 词： | 膀胱移行细胞癌 透明质酸 合酶 基因 生物学行为 |
The expression and significance of various genes of hyaluronic acid synthase isoforms in different stage/grade human bladder transitional cell carcinoma |
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| CAI Jianliang,XIN Dianqi,LV Tianjing,HE Qun,NA Yanqun.The expression and significance of various genes of hyaluronic acid synthase isoforms in different stage/grade human bladder transitional cell carcinoma[J].Journal of Clinical Urology,2008,23(5):348-351. |
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| Authors: | CAI Jianliang XIN Dianqi LV Tianjing HE Qun NA Yanqun |
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| Institution: | 1Department of Urology;Peking University Shougang Hospital;Beijing;100041;China;2Department of Urology;Peking University First Hospital;Institute of Urology |
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| Abstract: | Objective:To investigate the expression of hyaluronic acid synthase(HAS) family mRNAs in human bladder transitional cell carcinoma (BTCC) and its potential clinical value. Methods:The expression of all human hyaluronic acid synthase isoforms (HAS1, HAS2 and HAS3) mRNAs in 145 different stage/grade human BTCC, 22 human normal bladder mucosa tissue samples and different malignancy human BTCC cell lines (RT4, G1; BIU87, G2; EJ, G3 and T24, G3) were examined with semiquantitative RT-PCR. Results:We obtained all... |
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| Keywords: | Bladder neoplasms Hyaluronic acid Synthase Gene Biological |
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