血栓栓塞症患者抗活化的蛋白C的研究

目的 探讨抗活化的蛋白Ｃ（ＡＰＣＲ）现象在中国人血栓栓塞症发病中的作用。方法 用ＡＰＴＴ法检测ＡＰＣＲ敏感比率（ＡＰＣＲ－ＳＲ）来研究４０例深静脉血栓症（ＤＶＴ）患者、５２例脑血栓形成患者和１００例正常人的ＡＰＣＲ发生率；用多聚酶链反应－限制性内切酶长度多态性（ＰＣＲ－ＲＦＬＰ）来检测ＦＶ Ｌｅｉｄｅｎ突变；用免疫火箭电泳测血浆总蛋白Ｓ和游离蛋白Ｓ。结果 正常人的正常化ＡＰＣＲ－ＳＲ（ｎ－ＡＰＣＲ

Study of APC resistance in patients with thromboembolism

Objective To evaluate the effects of activated protein C resistance (APCR) in pathogenesis of thromboembolism in Chinese. Method: The incidence of APCR in 40 cases of deep venous thrombosis (DVT) patients, 52 cases of is chemic stroke and l00 normal controls were detected by activated partial thromboplastin time (APTT) in the Presence and absence of APC. FV Leiden was detected by PCR-RFLP assay.Plasma levels of TPS:Ag and FPS:Ag were measured by using rocket immunoelectrophoresis. Results: n-APCR-SR in normal controls was 0.93?.32 but there were six cases whose n-APCR-SR was below 0.61 both in 40 DVT patiods and in 52 ischemic stroke patients. The incidence of APCR in the patients was 15% and 11.5% respectively, which were significantly different from that of control group (P<0.05). No FV Leiden mutation was found in this study. There was no significant difference in Plasma PS Levels between the patients and controls. Conclusion APCR might be a risk factor in pathogenesis of thromboembolism and there existed the Plausible pathogenic divergence in the patients among different races.