束缚应激对S180荷瘤小鼠免疫功能的影响

目的 :观察束缚应激对S180荷瘤小鼠T、B淋巴细胞增殖能力和腹腔巨噬细胞吞噬功能的影响。方法 :取腹腔传代第 7天的S180肿瘤细胞 ,接种于昆明小鼠右腋皮下。接种后束缚限制活动 ,8小时 天。并设单纯束缚组、单纯肿瘤和正常对照组。 10天后处死小鼠 ,取脾脏检测T、B淋巴细胞增殖能力 ,抽取腹腔巨噬细胞测定吞噬功能 ,并剥取肿瘤称瘤重。结果 :束缚应激可明显降低S180荷瘤小鼠和正常小鼠脾T、B淋巴细胞增殖率 (P分别 <0 0 1和 <0 0 5 )和腹腔巨噬细胞吞噬能力 (P <0 0 1) ,并可明显增加肿瘤重量 (P <0 0 1)。结论 :束缚应激可抑制荷瘤小鼠和正常小鼠免疫功能 ,并可通过抑制荷瘤小鼠的免疫功能促进肿瘤生长。

Influence of Restraint Stress on the Immune Function of Mice with S180 Tumor

Objective:To investigate the effect of restraint stress on T, B lymphocyte transformation rate and phagocytic function of peritoneal macrophage.Method:Cells were extracted from the abdominal cavity of mice with S180 tumor and then were injected subcutaneously in the right oxter in 0.2 ml/mouse. Some of the mice were bound at the same time. There were four groups in all, they were normal control, tumor mice without restrain, restrained mice without tumor, and mice with both tumor cell injection and restrain. All mice were killed after 10 days, T and B lymphocyte transformation rates of spleen were examined, the phagocytic function of peritoneal macrophage was also tested. The weights of tumors were also recorded.Result:Compared with tumor mice without restrain, the mice with both tumor injection and restrain had lower T, B lymphocyte transformation rates and lower phagocytic function of peritoneal macrophage. The weight of tumor in the study group was also higher than that of tumor mice without restrain.Conclusion:Restraint stress aggravates the depression of the immune function of mice with S180 tumor and promotes the growth of the tumor.