CYP2C19 polymorphisms in patients with gastric and colorectal carcinoma |
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Authors: | Tamer L Ercan B Ercan S Ate? N Ate? C Ocal K Dirlik M Aydin S Atik U |
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Institution: | (1) Department of Bioistatistics, Mersin University, Turkey;(2) Department of Medical Biology and Genetics, Mersin University, Turkey;(3) Department of General Surgery, Mersin University, Turkey;(4) Department of Genernal Surgery, Kocaeli University, Turkey;(5) Department of Biochemistry, Mersin University, Medical Faculty, 33079 Mersin, Turkey |
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Abstract: | Background It has been reported that up to 80% of human cancer arise as a consequence of environmental exposure and host susceptibility
factors. Environmental carcinogens are predominantly metabolized by the cytochrome P450 (CYP) superfamily of drug-or xenobiotic-metabolizing
enzymes. Genetic variations in these enzymes affect individuals' susceptibility to carcinogens.
Aim of the study The aim of this study was to evaluate the relationship between CYP2C19 polymorphism and susceptibility to these cancers by
means of CYP2C19 genotyping among Turkish subjects.
Methods DNA of subjects were isolated from leukocytes by high pure template preparation kit (Roche Diagnostics, GmbH, Mannheim, Germany)
and genotypes were detected by LightCycler CYP2C19 Mutation Detection Kit by real-time PCR with LightCycler instrument (Roche Diagnostics, cat. no. 3113914).
Results Being male was associated with a 3.5-fold (OR: 4.27, CI: 2.27–8.05) and 4.27-fold (OR: 3.50, CI: 1.948–6.301) risk for colorectal
and gastric carcinoma, respectively. The CYP2C19
*
3 heterozygote genotype was not found in either gastric or colorectal carcinoma patients. Although the frequency of CYP2C19*2 heterozygote genotype is high in patients with gastric and colorectal carcinoma, it is not significantly associated with
cancer (OR: 1.79, CI: 0.829–3.865 and OR: 1.998, CI: 0.961–4.154, respectively).
Conclusion Although the frequency of CYP2C19
*
2 heterozygote genotype is high in our patients with gastric and colorectal carcinoma, there is no the relationship between
CYP2C19 polymorphism and susceptibility to these cancer. |
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Keywords: | CYP2C19 polymorphism gastric and colorectal carcinomas |
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