经导管动脉化疗栓塞术对原发性肝癌外周循环肿瘤细胞及血管内皮生长因子的影响

目的 探讨经导管动脉化疗栓塞术（TACE）对原发性肝癌患者外周循环肿瘤细胞（CTC）数量及血管内皮生长因子（VEGF）浓度的影响。方法 选择2011年至2013年50例原发性肝癌患者为研究对象，应用免疫磁珠分离技术及酶联免疫吸附法检测在TACE术前1天及术后1天、3天、30天CTC数量和VEGF浓度的变化。结果 TACE术前1天和术后1天、3天、30天的CTC阳性率分别为84.0％和66.0％、58.0％、78.0％，CTC检测数量分别为（2.38±1.65）个、（1.24±1.06）个、（0.86±0.83）个、（2.10±1.63）个，手术前后比较差异均有统计学意义（P＜0.05）；术前1天及术后1天、3天、30天的VEGF浓度分别为（304.98±33.94） pg／ml、（262.93±28.22） pg／ml、（258.40±27.55） pg／ml、（283.08±37.19） pg／ml，手术前后比较差异有统计学意义（P＜005）。对CTC数量及VEGF浓度在术后30天与术后3天两时段的差值进行相关分析，两者呈线性正相关（r=0.710，P＜0.05）。结论 TACE治疗原发性肝癌术后1～3天内可以有效减少血液VEGF浓度并阻止肿瘤细胞进入外周血循环。

Effect of transcatheter arterial chemoembolization on the circulating tumor cells number and the vascular endothelial growth factor concentration in patients with hepatocellular carcinoma

Objective To investigate the effect of transcatheter arterial chemoembolization（TACE）treatment on the circulating tumor cell（CTC） number and the serum vascular endothelial growth fator（VEGF） concentration in patients with primary liver cancer（PLC）. Methods Totally fifty patients with primary PLC were select from the First Affiliated Hospital of Baotou Medical College between 2011 and 2013.The CTC number and VEGF concentration on day 1 before TACE， as well as day 1， day 3 and day 30 after TACE were measured by immunomagnetic beadssparation techniques（IMBS） and enzyme linked immunosorbent assay（ELISA）. Results The positive rates of CTC on day 1 before TACE， as well as day 1， day 3 and day 30 after TACE were 84.0％， 66.0％， 58.0％ and 78.0％， respectively. The CTC numbers were 2.38±1.65， 1.24±1.06， 0.86±0.83， 2.10±1.63， respectively. The difference was statistically significant before and after TACE （P＜0.05）. The concentration of VEGF on day 1 before TACE， as well as on day 1， day 3 and day 30 after TACE were （304.98±33.94） pg／ml and （262.93±28.22） pg／ml，（258.40±27.55） pg／ml，（283.08±37.19） pg／ml. The difference was statistically significant before and after TACE （P＜0.05）. Conclusion The VEGF concentration and CTC number will reduce within 1-3 days after TACE in patients with PLC.