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| 蜂胶中总黄酮的含量测定 | |
| 引用本文： | 李彩霞,班桂荣,董玉. 蜂胶中总黄酮的含量测定[J]. 中国药业, 2010, 19(21): 10-11 |
| 作者姓名： | 李彩霞 班桂荣 董玉 |
| 作者单位： | 1. 内蒙古医学院附属人民医院药剂科,内蒙古,呼和浩特,010020 2. 内蒙古锡林郭勒盟白旗医院,内蒙古,锡林郭勒,013800 3. 内蒙古医学院药学院,内蒙古,呼和浩特,010059 |
| 摘 要： | 目的建立测定蜂胶中总黄酮含量的紫外分光光度法。方法以芦丁为对照品,采用紫外分光光度法测定蜂胶中总黄酮含量,检测波长为528nm。结果芦丁质量浓度在7.952～39.76μg/mL范围内与吸光度呈良好线性关系,线性回归方程为Y=8.3124X＋0.0217（r=0.9996）,平均回收率为101.85%,RSD为2.31%（n=6）。结论该方法简便、灵敏、准确,可用于蜂胶中总黄酮的含量测定。 |
| 关 键 词： | 蜂胶 总黄酮 紫外可见分光光度法 含量测定 |
Determination of Total Flavonoids in Propolis |
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| Li Caixia,Ban Guirong,Dong Yu. Determination of Total Flavonoids in Propolis[J]. China Pharmaceuticals, 2010, 19(21): 10-11 | |
| Authors: | Li Caixia Ban Guirong Dong Yu |
| Affiliation: | 1.Department of Pharmacy,Affiliated People＇s Hospital,Neimenggu Medical College,Huhhot,Inner Mongolia,China 010020;2.Neimenggu Baiqi Hospital,Xilingol,Inner Mongolia,China 013800;3.Department of Pharmacy,Neimenggu Medical College,Huhhot,Inner Mongolia,China 010059) |
| Abstract: | Objective To establish a content determination method of total flavones in propolis.Methods With rutin as the reference substance,the UV-visible spectrophotometry was used to determine flavonoids in propolis.The detection wavelength was 528 nm.Results Rutin had the good linear relationship with the concentration in the range of 7.952-39.76 μg /mL and the regression equation was as follows：Y = 8.312 4 X ＋ 0.021 7（r = 0.999 6）.The average recovery rate was 101.85%,RSD 2.31%（n = 6）.Conclusion The method is simple,sensitive and accurate,which can be used for the total flavones determination in propolis. |
| Keywords: | propolis total flavones UV-visible spectrophotometry content determination |
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