紫外线照射充氧自血回输疗法对兔脊髓损伤后钙含量的影响

目的：观察兔脊髓损伤后钙含量动态变化，研究紫外线照射充氧自血回输疗法治疗脊髓损伤的机理。方法：用Ａｌｅｎ氏法造成兔脊髓损伤，用离子选择电极法，原子吸收光谱法，钙组织化学染色法测定脊髓损伤后钙含量变化。结果：损伤组血清中离子钙含量升高；脊髓组织中损伤区总钙及细胞内钙含量升高，而邻近区脊髓组织总钙含量升高较迟，幅度较小，治疗后下降；血清中总钙含量治疗前后无变化。结论：紫外线照射充氧自血回输疗法能够改善兔脊髓损伤后离子失衡状态，拮抗钙离子内流，从而能减轻脊髓继发性损伤。

Effect of ultraviolet blood irradiation and oxygenation on calcium level after spinal cord injury in rabbits

Objective: In order to observe the dynamic alteration of calcium level after spinal cord injury and study the mechanism of ultraviolet blood irradiation and oxygenation (UBIO) on spinal cord injury. Methods: The spinal cord injuries of rabbits were incurred by Allen's method.The calcium level of injuried spinal cord was respectively measured by ion-selected electrode,atomic absorption spectroscopy and calcium histochemical stain. Results: In spinal cord injury group, the Ca2 of serum,the calcium level of spinal injuried area and intracellular was increased, but it was delayed in adjacent spinal cord.However,the manifestations were improved after UBIO therapy .The total calcium level of serum was unchanged before and after UBIO treatment. Conclusion: It indicates that UBIO can regulate the balance of Ca2 ,antagonize the inflow of calcium and alleviate the secondary injuries for spinal cord injury.