Mussel-Inspired Carboxymethyl Chitosan Hydrogel Coating of Titanium Alloy with Antibacterial and Bioactive Properties |
| |
Authors: | Yanru Ren Xiaoyan Qin Mike Barbeck Yi Hou Haijun Xu Luo Liu Chaoyong Liu |
| |
Institution: | 1.Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, China; (Y.R.); (X.Q.); (Y.H.); (H.X.);2.Institute of Material Science and Technology, Technical University of Berlin, Hardenbergstrasse 40, Sekr. BA3, 10623 Berlin, Germany; |
| |
Abstract: | Infection-related titanium implant failure rates remain exceedingly high in the clinic. Functional surface coating represents a very promising strategy to improve the antibacterial and bioactive properties of titanium alloy implants. Here, we describe a novel bioactive surface coating that consists of a mussel-inspired carboxymethyl chitosan hydrogel loaded with silver nanoparticles (AgNPs) to enhance the bioactive properties of the titanium alloy. The preparation of hydrogel is based on gallic acid grafted carboxymethyl chitosan (CMCS-GA) catalyzed by DMTMM (4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride). To build a firm bonding between the hydrogel and titanium alloy plate, a polydopamine layer was introduced onto the surface of the titanium alloy. With HRP/H2O2 catalysis, CMCS-GA can simply form a firm gel layer on the titanium alloy plate through the catechol groups. The surface properties of titanium alloy were characterized by scanning electron microscope (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle. Silver nanoparticles were loaded into the gel layer by in situ reduction to enhance the antibacterial properties. In vitro antibacterial and cell viability experiments showed that the AgNPs-loaded Ti-gel possesses excellent antibacterial properties and did not affect the proliferation of rabbit mesenchymal stem cells (MSCs). |
| |
Keywords: | carboxymethyl chitosan gallic acid titanium alloy DMTMM HRP/H2O2 catechol group hydrogel coating antibacterial |
|
|