重组人FLT3配基对人脐血CD34＋细胞的体外扩增作用

目的观察重组人ＦＬ（ｒｈＦＬ）对脐血ＣＤ＋３４细胞的体外扩增作用。方法用磁性细胞分离仪富集人脐血ＣＤ＋３４细胞。在ＣＤ＋３４细胞体外液体培养中加入不同的细胞因子,在不同时间取样测试ＣＤ＋３４细胞阳性百分率、晚期造血祖细胞（粒细胞巨噬细胞集落生成单位及红系爆增式集落形成单位）并计数细胞总数。结果用磁性细胞分离仪可快速富集脐血ＣＤ＋３４细胞,纯度高达８０％以上。ｒｈＦＬ可协同白细胞介素（ＩＬ）－３、ＩＬ－６、粒－巨噬细胞集落刺激因子及促红细胞生成素促进ＣＤ＋３４细胞总数在７天时扩增３．４倍,无ｒｈＦＬ的对照组ＣＤ＋３４细胞只扩增１．５倍,但对晚期造血祖细胞的扩增无明显协同作用。结论用排除法证实ｒｈＦＬ主要扩增了早期造血祖细胞。

Effect of recombinant human FLT3 ligand (rhFL) on in vitro expansion of human cord blood CD34+ cells

Objective To determine the effects of recombinant human FLT3 ligand (rhFL) on in vitro expansion of human cord blood CD 34 cells. Methods Human cord blood CD 34 cells were enriched by using a magnetic cell isolation system. CD 34 cells were incubated in liquid culture in the presence of different cytokines. The total number of all cells and the number of CD 34 cells and hematopoietic progenitor cells (CFU GM and BFU E) were counted at various time intervals. Results Human cord blood CD 34 cells could be enriched to the purity of more than 80%. The number of CD 34 cells increased 3.4 folds in the presence of rhFL IL3 IL6 GMCSF EPO in seven days, while in the control group without rhFL, it increased 1.5 folds. The difference of the number of more matured hematopoietic progenitor cells in the CD 34 cell population between the control group and the rhFL group was not significant. Conclusion Only the primitive hematopoietic progenitor cells are expanded by rhFL according to the results obtained in the exclusion method.