Effect of norcantharidin on the proliferation,apoptosis, and cell cycle of human mesangial cells |
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| Authors: | Kun Ye Qiaoyu Wei Zhifeng Gong Yunfeng Huang Hong Liu Ying Li |
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| Affiliation: | 1. Department of Nephrology, The People’s Hospital of Guangxi Zhuang Autonomous Region, Guangxi, China;2. Department of Nephrology, The Second Xiangya Hospital of Central South University, Hunan, China |
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| Abstract: | Aims: Norcantharidin (NCTD) regulates immune system function and reduces proteinuria. We sought to investigate the effect of NCTD on proliferation, apoptosis and cell cycle of cultured human mesangial cells (HMC) in vitro.Methods: HMC cells were divided into a normal control group, and various concentrations of NCTD group (2.5, 5, 10, 20, or 40?μg/mL). Cell proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, apoptosis was detected by Annexin V/propidium iodide (PI) assays, and morphological analysis was performed by Hoechest 33258 staining. Finally, cell cycle was analyzed by flow cytometry.Results: NCTD dose and time dependently inhibits HMC proliferation significantly (p?Conclusion: NCTD inhibits HMC cell proliferation, induces apoptosis, and affects the cell cycle. |
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| Keywords: | Mesangial cell norcantharidin proliferation apoptosis cell cycle |
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