| GAD_(67)-GFP基因敲入小鼠脊髓背角GABA能神经元与不同5-HT受体亚型的共存研究 |
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| 引用本文： | 王亚云,黄静,汪伟,武胜昔,李云庆.GAD_(67)-GFP基因敲入小鼠脊髓背角GABA能神经元与不同5-HT受体亚型的共存研究[J].神经解剖学杂志,2008,24(4):338-342. |
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| 作者姓名： | 王亚云 黄静 汪伟 武胜昔 李云庆 |
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| 作者单位： | 第四军医大学基础医学部人体解剖学教研室暨梁球琚脑研究中心,西安710032 |
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| 摘 要： | 为初步探讨5-HT受体亚型对脊髓背角内抑制性GABA能神经元的介导作用,本研究以GAD67-GFP基因敲入小鼠为工具,利用免疫荧光双标记方法,检测了5-HT受体亚型与脊髓背角GABA能阳性神经元的共存情况。结果显示,GABA能阳性细胞与5-HT1A,5-HT2A和5-HT3等受体亚型共存,且共存率存在明显不同。以上结果提示5-HT受体各亚型在脊髓水平发挥不同的作用,从而参与完成5-HT复杂的生理效应。
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| 关 键 词： | 5-羟色胺 γ-氨基丁酸 5-羟色胺受体亚型 脊髓背角 免疫荧光组织化学 |
Colocalization of 5-HT receptors on the gabaergic neurons within the spinal dorsal horn of GAD67-GFP knock-in mice |
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| Abstract: | In order to elucidate the mediating role of 5-HT receptor subtypes on GABAergic transmission in the spinal cord dorsal horn,in the present study,the double immunofluorescent stainging is used to detect the expression patterns of serotonin(5-HT)receptors on the γ-Aminobutyric acid(GABA)neurons within the spinal dorsal horn of glutamic acid decarboxylase 67(GAD67)-green fluorescent protein(GFP)knock-in mice.Results from these two methods were similar:GABAergic neurons express 5-HT1A,5-HT2A and 5-HT3A receptor subtypes.Our results indicate that different 5-HT receptor subtypes function variously,and thus participate the complicated physiological effects of 5-HT at the spinal level. |
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| Keywords: | Serotonin γ-aminobutyric acid serotonin receptor subtypes spinal dorsal horn immunofluroscence histochemistry |
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