粉防己碱抑制激光诱导鼠脉络膜新生血管

目的 观察玻璃体腔注射粉防己碱对实验性鼠脉络膜新生血管的抑制作用及其对视网膜结构和功能的影响。 方法 应用半导体激光（波长810 nm，曝光时间0.1 s，光斑直径100 μm，能量120 mW）光凝诱导20只Brown Norway (BN) 大鼠20只眼的脉络膜新生血管(CNV)模型。将大鼠随机分为实验组和对照组，每组各10只大鼠20只眼，实验组大鼠激光光凝后0、3 d玻璃体注射0.05 ml浓度为3.21 μmol/L的粉防己碱药液，对照组玻璃体内注射同体积的生理盐水；两组均在激光光凝14 d后行荧光素眼底血管造影，观察其新生血管的发生率。另有5只健康BN大鼠，每只鼠右眼玻璃体内注射入0.05 ml浓度为3.21 μmol/L的粉防己碱药液，左眼注射同体积生理盐水，第一次注药前及注药后1 h、1 d和第二次注药后1 h、1、7、14 d行视网膜电图（ERG）检查，第二次注药后14 d行光学、电子显微镜检查。 结果 实验组大鼠CNV发生率为23.26%，明显低于对照组63.33%（P<0.01）。3.21 μmol/L的粉防己碱玻璃体内注射b波波幅比率与注药前相比差异无统计学意义(P>0.05)。光学、电子显微镜检查未见明显细胞异常。 结论 粉防己碱能抑制鼠CNV形成；3.21 μmol/L浓度的粉防己碱玻璃体内注射对视网膜无毒性作用。 (中华眼底病杂志, 2006, 22, 242-244)

Laser-induced choroidal neovascularization inhibited by tetrandrine in rats

Objective To investigate the effect of tetrandrine (Tet) on experimental choroidal neovascularization and the effect of Tet on retinal structure and function. Methods Choroidal neovascularization was induced in 20 Brown Norway (BN) rats (40 eyes) by diode laser (wavelength: 810 nm; exposal time: 0.1 second; facular diameter:100 μm; energy: 120 mW), and the rats were divided randomly into experimental and control group with 10 rats (20 eyes) in each group. In experimental group, 0.05 ml Tet with the concentration of 3.21 μmol/L was injected intravitreously 0 and 3 days after laser photocoagulation; in the control group, the rats underwent an intravitreous injection with the same volume of sodium chloride solution. The incidence of CNV was evaluated by fundus fluorescein angiography (FFA) 14 days after laser photocoagulation. Five right eyes of another Five healthy BN rats underwent intravitreous injection with 0.05 ml Tet with the concentration of 3.21 μmol/L, and an intravitreous injection with the same volume of sodium chloride solution was performed on the left eyes. Before injection, 1 hour, and 1 day after the first injection, and 1 hour, 1 day, 7 days, 14 days after e second injection the electroretinography (ERG) was performed on these 5 rats; 14 days after the second injection, the retinae were examined by light microscopy and transmission electron microscopy. Results The incidence of CNV was 23.26% in experimental group，which was obviously lower than that in the control group (63.33%) (P<0.01). The ratio of amplitude of b wave of ERG in the rats undergone intravitreous injection with 3.21 mg/ml Tet didn′t differ much from which before the injection (P>0.05). There were no structural changes of retinal tissues examined by light and electron microscopy. Conclusion Tet may inhibit choroidal neovascularization in rats; there isn′t any significant toxic effect of intravitreous injection with Tet on retina at the dosage of 3.21 μmol/L.  (Chin J Ocul Fundus Dis, 2006, 22: 242-244)