老龄大鼠非细菌性多器官功能不全综合征模型的建立

目的以酵母多糖腹腔注射建立老龄大鼠多器官功能不全综合征(MODSE)模型,并进一步与成年多器官功能不全综合征(MODS)模型作比较.方法成年和老龄SD大鼠,腹腔注射不同剂量酵母多糖,以血气、血生化和心、肝、脑、肺、肾等各主要脏器的光镜检查等为指标,观察上述各主要脏器的功能和形态学的变化.结果与成年和老龄正常对照组相比,成年和老龄不同酵母多糖组血气和血生化指标均出现不同程度的改变,肺、心、肝、脑、肾等主要脏器出现明显病理改变.在相同酵母多糖浓度下,老龄组呼吸功能、心脏功能和肾脏功能降低较相应成年组重;在复制模型程度相似的情况下,与成年大鼠相比,老龄大鼠有死亡时间延迟的趋势.结论酵母多糖腹腔注射可复制MODSE模型,以0.5 g/kg腹腔注射最为理想,该模型可用于MODSE机制和实验治疗学的探讨.

A model of non-bacterial multiple organ dysfunction syndrome in the elderly rats

Objective To establish a mod el of multiple organ dysfunction sydrome in the elderly (MODSE) rats by intraperit oneal injection of different doses of zymosan, and to compare the multiple organ dysfunction syndrome (MODS) in the adult and in the elderly. Methods Intraperitoneal injection of different doses of zymosan was given to the adult and senile rats and the changes of the function and morphology of vital or gans including the heart, liver, brain, lungs, and kidneys were observed by the an alysis of blood gas, blood biochemistry as well as the histopathologic examinati on. Results Compared with the normal adult and elderly rat contro ls, the blood gas and blood biochemistry changed to different extents in the zym osan groups of different doses. The pathological changes were also observed in the vital organs including the lungs, heart, liver, brain, kidneys, etc. After i nj ection of the same concentrations of zymosan, the reductions of respiratory, car diac and renal functions in the senile groups were much more severe than those i n the corresponding adult group. In the duplicate model of similar degree, the senile rats showed the tendency to die later than the adult rats. Conclu sion Zymosan can be used in elderly animal model to incite MODS with the best dosage of 0.5 g/kg and thus the model can be used to study the mechanisms a nd the experimental therapeutics of MODSE.