| 肺炎衣原体套式聚合酶链式反应检测研究 |
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| 引用本文： | 秦玲,糜祖煌,陆亚华. 肺炎衣原体套式聚合酶链式反应检测研究[J]. 中国人兽共患病杂志, 2000, 16(2): 84-86 |
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| 作者姓名： | 秦玲 糜祖煌 陆亚华 |
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| 作者单位： | [1]无锡市克隆遗传技术研究所 [2]常州市第一人民医院儿科 |
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| 基金项目： | 本文已被国际抗痨联盟第20届东区会议录用,并由香港胸肺基金会资助与会(A-107)。 |
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| 摘 要： | 目的 探讨肺炎衣原体（Ｃｐｎ）的套式聚合酶链式反应（ｎＰＣＲ）检测技术。方法 根据Ｃａｍｐｂｅｌｌ克隆的ＣｐｎＤＮＡ序列设计套式引物,并建立ｎＰＣＲ反应体系。结果 Ｃｐｎ经ｎＰＣＲ扩增出现３７８ｂｐ的阳性条带,而沙眼衣原体和鹦鹉热衣原体以及其他用作特异性试验的微生物均不能扩增。３例阳性标本经ＤＮＡ测序与Ｃｐｎ（ＣＷＬ－２９株）序列完全一致。Ｃｐｎ ｎＰＣＲ之灵敏度比传统ＰＣＲ高１００倍。各种呼吸系
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| 关 键 词： | 肺炎衣原体 聚合酶链反应 nPCR 诊断 |
| 文章编号： | 1002-2694(2000)02-0084-02 |
DETECTION OF CHLAMYDIA PNEUMONIAEBY NESTED POLYMERASE CHAIN REACTION |
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| QIN Ling,MI Zuhuang,LU Yahua. DETECTION OF CHLAMYDIA PNEUMONIAEBY NESTED POLYMERASE CHAIN REACTION[J]. Chinese Journal of Zoonoses, 2000, 16(2): 84-86 |
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| Authors: | QIN Ling MI Zuhuang LU Yahua |
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| Abstract: | Aim To develop a molecular biologic technique for detection of chlamydia pneumoniae with nested polymevase chain reaction (nPCR) .Methods Nested primers were synthesized according to a cloned C.pneumoniae 474 - bp Pst Ifragmert.Results The 378be DNA fragments were amplified from C.pneuomoniae with nPCR.Nere of the C.trachomatis,C.psittaci, other organisms and etc.Strains tested were amplified by the nPCR.The sequening of 3 sample products with nPCR are quite same as C. pneumoniae (CWL - 29). The sensitivity of nPCR is higter than PCR.Conclusions This method is not only sensitive. specific and rapid but also provides an etiological basis fro diaghosis of C.pneumoniae infection. |
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| Keywords: | Chlamydia pneumoniae Nested polymerase chain reaction |
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