HPVDNA永生化人宫颈上皮细胞的生长方式与宫颈上皮内肿瘤的比较

目的：研究人乳头瘤病毒（HPV）DNA永生化人宫颈上皮细胞在器官培养的生长特点及将其与体内宫颈上皮内肿瘤（CIN）进行比较。方法：先用HPV16和18型DNA转染人宫颈上皮细胞，建立永生化的人宫颈上皮细胞株，再用胶原筏培养方法分析永生化人宫颈上皮细胞在器官培养的生长特点，将其与体内CIN的形态进行了比较。结果：人宫颈上皮细胞经HPV16和18DNA转染后，可以使其变成一种永生化细胞。细胞生物学研究显示永生化细胞是一种前恶性细胞。胶原筏培养显示水生化人宫颈上皮细胞的生长行为类似体内CIN。结论：HPV感染主要影响宫颈癌的发生的早期阶段。

Comparison of growth behavior between HPV DNA-immortalized human cervical epithelial cells and cervical intraepithelial neoplasia

Purpose To study the growth behaviors of the high-risk HPV DNA-immortalized human cervicalepithelial cells in the organic culture and compare them with cervical intraepithelial neoplasia (CIN) in vivo.Methods At first the full-length HPV 16 and 18 DNAs were trans feeted into the primary human cervical ep-ithelial cells by the gene transfection technique. The growth behaviors of the HPV DNA-immortalized humancervical epithelial cells on collagen raft culture were characterized and compared with the growth features ofCIN. Results The four HPV DNA-immortalized human cervical epithelial cell lines were established. Theywere shown premalignant cell properties on basis of the studies of the cell biology. The growth behaviors of HPVDNA-immortalized human cervical epithelial cells on collagen raft culture resembled that of CIN. ConclusionThe results suggest that HPV 16 and 18 infections play an important role in the early stages of cervical epithelialcarcinogenesis.