紫杉醇脂质体对宫颈癌HeLa细胞作用的实验研究

目的探讨注射用紫杉醇脂质体对人宫颈癌细胞系的杀伤作用。方法以0．01μmol／L、0．1μmol／L、1μmol／L、10μmol／L和100μmol／L浓度梯度的含注射用紫杉醇脂质体培养基作用于人宫颈癌细胞系HeLa细胞，并设对照（普通培养基，不含药物），通过MTT、显微镜观察细胞形态、流式细胞分析技术检测该药对细胞生长的影响；以Western blot分析细胞外信号调节激酶（ERK）、磷酸化细胞外信号调节激酶（P—ERK）蛋白表达情况。结果经上述不同浓度紫杉醇脂质体作用48h后，细胞存活率下降；镜下观察显示部分细胞皱缩变形，崩解坏死，细胞脱壁悬浮；流式细胞结果显示细胞凋亡率逐渐增加，分别为（14．16±4．78）％、（43．68±16．21）％、（63．70±3．74）％、（63．51±2．29）％、（72．84±10．77）％和（74．69±7．52）％；细胞周期各时相中，S期比例减少；Western blot显示HeLa细胞的P—ERK蛋白表达逐渐减弱（0．7645±0．0198、0．7592±0．0072、0．2655±0．0356、0．3232±0．0229、0．1467±0．0091）。结论紫杉醇脂体可抑制宫颈癌HeLa细胞的生长，对HeLa细胞的作用可能通过抑制MAPK信号转导通路达到抑制肿瘤的目的。

Effect of liposome encapsulated paelitaxel on cervical carcinoma cells in vitro

Objective To investigate the effect of liposome encapsulated paclitaxel on human cervical carcinoma HeLa cells in vitro. Methods HeLa cells were incubated with different concentrations of liposome encapsulated paclitaxe （0.01 μmol/L, 0.1 μmol/L, 1 μmol/L, 10 μmol/L, 100 μmol/L）. The growth of the cells were measured by MTT assay and flow eytometry assay （FCM）. The cell morphology changes were observed under microscope. The expression of ERK and P - ERK were detected by Western blot. Results After 48 hours treatment of liposome encapsulated paclitaxel, cellular degeneration and necrosis were found. With the increase of the concentration of paelitaxel, the proportion of apoptotic cells were increased （14. 16 ±4. 78） %, （43. 68 ± 16. 21 ） %, （ 63.70 ± 3.74 ） %, （63.51 ± 2.29）%, （72.84 ± 10.77）% and （74.69± 7.52） %, respectively. Proportion of cells at S phase decreased. The expression of phosphorylated ERK decreased （0. 764 5±0. 019 8, 0. 759 2±0. 007 2, 0. 265 5±0. 035 6, 0. 323 2±0. 022 9, 0. 146 7±0. 009 1）. Conclusion Liposome encapsulated paclitaxel can suppress growth of the cervical carcinoma cells, probably via inactivation of the MAPK signal transduction pathway.