| 人工牛黄蛋白质的凝胶电泳实验研究 |
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| 引用本文： | 殷丹,陈振江,刘焱文.人工牛黄蛋白质的凝胶电泳实验研究[J].湖北中医学院学报,2006,8(1):42-44. |
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| 作者姓名： | 殷丹 陈振江 刘焱文 |
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| 作者单位： | 湖北中医学院 湖北武汉430061 |
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| 摘 要： | 目的用凝胶电泳建立对人工牛黄蛋白质的研究方法。方法利用IFE、SDS-PAGE、PAGE技术,进行牛黄中蛋白质的电泳实验。结果得到清晰的电泳图谱。结论该方法准确、可靠、重复性好,可对牛黄及其制剂的鉴别提供重要参数。
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| 关 键 词： | 牛黄 IFE SDS-PAGE PAGE 等电PH |
| 文章编号： | 1008-987X(2006)01-0042-03 |
| 收稿时间： | 06 12 2005 12:00AM |
| 修稿时间： | 2005年6月12日 |
Studies on Gel Electrophoresis of Proteins of Calculus Bovis |
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| YIN Dan,CHEN Zhen-jiang,LIU Yan-wen.Studies on Gel Electrophoresis of Proteins of Calculus Bovis[J].Journal of Hubei College of Traditional Chinese Medicine,2006,8(1):42-44. |
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| Authors: | YIN Dan CHEN Zhen-jiang LIU Yan-wen |
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| Abstract: | Objective:To establish the method of proteins of Calculus Bovis by gel electrophoresis.Methods:Horizontal gel is prepared by using Amphline and a crylamide and the condition of electrophoresis is optimized.Results:there were two types of proteins of Calculus Bovis.Conclusion:This method is accurate,reliable,and with good reproducibility.It can be applied to important factors of Calculus Bovis and the preparation. |
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| Keywords: | Calculus Boris IFE SDS-PAGE PAGE Isoelectric PH |
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