鼻腔、鼻窦内翻性乳头状瘤癌变过程PCNA和PTEN蛋白表达的意义

目的研究鼻腔、鼻窦内翻性乳头状瘤癌变过程增殖细胞核抗原(Proliferating Cell Nuclear Antigen,PCNA)和与张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶基因(phosphatase and tensin ho-mology deleted on chromosome ten,PTEN)蛋白的表达,并探讨其与乳头状癌发生的关系。方法应用免疫组化EnVision二步法,对55例鼻腔、鼻窦内翻性乳头状肿瘤石蜡标本进行PCNA和PTEN蛋白表达的检测。结果PCNA和PTEN在鼻腔、鼻窦内翻性乳头状肿瘤细胞均有表达,两者在细胞癌变过程显示不同的分布模式,即PCNA蛋白表达逐渐升高,而PTEN蛋白表达则逐渐下降。结论PCNA和PTEN蛋白表达的改变与鼻腔、鼻窦内翻性乳头状瘤的恶性转化密切相关,PCNA表达增加和PTEN表达缺失可能是一个有用的生物学标志和提示乳头状瘤病人预后的指标。

THE SIGNIFICANCE OF THE CHANGE OF PCNA AND PTEN PROTEIN EXPRESSION IN CARCINOGENESIS OF INVERTED PAPILLOMA OF NASAL CAVITIES AND PARANASAL SINUSES

Objective To investigate the expression of proliferating cell nuclear Antigen （PCNA） and assisted with the tension of protein and protein homology, the No. 10 chromosome lost phosphatase gene （phosphatase and tensin homology deleted on chromosome ten ,PTEN）in inverted papilloma of nasal cavities and paranasal sinuses and its carcinogenesis. Methods PCNA and PTEN protein expression in 55 paraffim tissue specimen of nasal cavities and paranasal sinuses,including inverted papilloma, atypical hyperplasia and papillary carcinoma were detected using immunohistochemical EnVision two step method. Results PCNA and PTEN protein were expressed in inverted papilloma, atypical hyperplasia and papillary carcinoma. The expression of PTEN protein showed a different distribution pattern to that of PCNA, the PCNA protein expression have a rising gradually and PTEN protein expression is decreasing gradually. Conclusions PCNA and PTEN protein expression change are closely related to the malignant transformation of inverted papilloma. PCNA expression up - regulated and loss of PTEN expressions may be a favorable marker and prompted prognosis in patient with inverted papilloma of nasal cavities and paranasal sinuses.