淫羊藿苷(ICA)对化疗后免疫抑制小鼠的免疫促进作用

目的:通过观察淫羊藿苷(ICA)对化疗药环磷酰胺(Cy)所致免疫抑制小鼠免疫功能的影响,探讨ICA促进免疫功能的作用及机理.方法:除正常组小鼠外,所有小鼠经腹腔注射Cy(300 mg/kg);第二天开始给予实验组小鼠灌胃不同剂量的ICA[150、80、40 mg/(kg·d)],阳性对照组小鼠尾静脉注射参芪扶正注射液(1 ml/d),模型组给予等量生理盐水,连续干预10天.所有小鼠均于末次给药12小时后处死,计算胸腺指数(TI)和脾指数(SI),光镜下计数外周血和脾淋巴细胞数量.MTT法检测小鼠脾淋巴细胞增殖反应.ELISA法检测TNF-α的含量.乳酸脱氢酶实验(LDH)检测小鼠脾脏NK和CTL细胞的活性.流式细胞术(FACS)检测脾淋巴细胞中NKT和CD3+T细胞的比例.结果:模型组小鼠以上各项免疫指标均有所下降.ICA处理组小鼠脾脏指数和胸腺指数均升高(P<0.01),脾淋巴细胞数量显著增加(P<0.01),但未达到正常对照组水平;ICA明显提高小鼠脾淋巴细胞的增殖反应,促进了小鼠脾NK和CTL细胞对肿瘤细胞的杀伤活性(P<0.01),提高了小鼠脾细胞TNF-α的产生(P<0.01).ICA处理组小鼠脾细胞中CD3+T、NKT细胞比例明显增加(P<0.01).结论:ICA能促进小鼠免疫功能,具有逆转化疗后小鼠免疫抑制状态的作用.

Immunoenhancement effect of ICA on the mouse model of Cy-induced hypoimmunity

Objective:Effect of ICA (Icariin) was observed on immunosuppression mice caused by Cyclophosphamide(Cy).Methods:A total of 60 mice,expect control group mice,were injected Cy intraperitoneally [300 mg/(kg·d)],then randomly divided into modelgroup(saline),positive (shenqi,1 ml/d)groups,ICA groups[150,80,40 mg/(kg·d)].Saline,shenqi and ICA were perfused respectively for 10 successive days;All mice were sacrificed after 12 h of last administration for performing following experiments.Measuring SI and TI and calculating amounts of lymphocyte from spleen and peripheral blood;Proliferative reaction of splenic lymphocyte stimulated by ConA and LPS were detected by MTT assays.Killing activity of NK and CTL cells for colon26 and level of TNF-α production were measured by LDH method.Ratios of CD3~+ T and NKT in splenic lymphocyte (SPL) were studied by FACS.Results:ICA can recovery TI and SI of mice to normal level and boost the population of splenic lymphocyte (SPL) (P<0.01).After treatment with ICA,activities of NK cell and CTL cell were significantly improved,and the level of TNF-α was significantly elevated.The ratios of NKT and CD3+T in the ICA-treated group were increased obviously (P<0.01).Conclusion:ICA can enhance immunological function and alleviate the side effect of chemotherapy effectively.