缺血预适应对肢体缺血再灌注大鼠肝脏的保护效应

目的 观察缺血预适应(IPC)对大鼠肢体缺血再灌注后肝脏损伤的影响，以进一步探讨IPC对肢体缺血再灌注后肝脏功能的保护作用。方法 实验用雄性Wistar大鼠18只，随机分为对照(Control)组，缺血再灌注(IR)组和缺血预处理(IPC IR)组．每组6只。分别测定血浆谷草转氧酶(ALT)、谷丙转氨酶(AST)、乳酸脱氢酶(LDH)．血浆和肝组织超氧化物歧化酶(SOD)、黄嘌呤氧化酶(XOD)、丙二醛(MDA)的含量变化及肝组织的湿/干重比值(W／D)、髓过氧化物酶含量(MPO)及DNA双链百分率(Ratio of DNA Chain％)。结果 发现IPC减轻了肢体IR后引起的ALT、AST、LDH、XOD、MDA、MPO、W／D含量的升高．并且增加了SOD以及肝组织中DNA双链百分率。结论 IPC对肢体IR继发的肝脏功能损伤具有保护作用。

THE EFFECT OF ISCHEMIA PRECONDITIONING ON THE INJURY OF LIVER FUNCTION AFTER LIMB ISCHEMIA-REPERFUSION IN RATS

Objective To observe the effect of ischemia pretreatment and investigate the protective effect of IPC on the injury of liver function after limb IR conditioning (IPC)on the injury of liver function after limb ischemia.Methods Using limb ischemia reperfusion injury model rats.Animal were randomly divided into three groups:control group,IR group and IPC group.The values of ALT?AST and LDH in the plasma were observed.The contents of superoxide dismutase (SOD),xanthin oxidase(XOD),malondialldehyde(MDA) in the plasma and the liver were measured.The levels of wet/dry ratio(W/D),myeloperoxidase(MPO)and ratio of DNA chain % in the liver were determined.Result It was found that IPC attenuated the increase contents of ALT?AST?LDH?XOD?MDA?MPO and W/D.It was also found that IPC increased the values of SOD?Ratio of DNA chain%.Conclusion IPC protected liver function from limb ischemia reperfusion injury in rats.