姜黄素与顺铂联合对人肺腺癌A549细胞增殖和凋亡作用

目的:研究姜黄素、顺铂和两者联合应用对人肺癌A549细胞增殖、凋亡和细胞周期的影响。方法:采用MTT法观察姜黄素和顺铂对人肺癌A549细胞增殖的影响;采用流式细胞仪检测姜黄素、顺铂和两者联合应用对人肺癌A549凋亡和细胞周期的影响。结果:①姜黄素及顺铂能抑制人肺腺癌A549细胞的增殖,并呈浓度及时问依赖性;姜黄素10,15,20μmol/L分别与顺铂1,2 mg/L联合24,48,72 h的抑制率显著高于单用顺铂、姜黄素组(均为P<0.05),两者呈现出相加的抗瘤效果。②姜黄素和顺铂均可在一定程度上诱导细胞凋亡,并且凋亡率与浓度呈正相关。两种药物联用后,诱导细胞凋亡作用显著增强。③姜黄素将细胞周期阻滞在G2/M期(P<0.01),顺铂将细胞周期阻滞在S期(P<0.01)。结论:姜黄素、顺铂均可抑制人肺癌A549细胞的增殖、诱导细胞的凋亡,在一定浓度范围内,呈量-效关系,而且两者联合应用具有相加或协同作用。

Effects of Curcumin Combined with Cisplatin on the Proliferation and Apoptosis of Human Lung Cancer Cell Line A549 in Vitro

Objective: To study the effect and possible mechanism of curcumin(CUR),cisplatin(DDP) and their synergergistic effect on the growth inhibition ratio,apoptosis and cell cycle distribution of human lung adenocarcinoma cell line A549.Methods: MTT was used to measure inhibitory effects of CUR and DDP on the growth of A549 cells.Flow cytometry was used to investigate the apoptosis and cell cycle distribution of A549 cells effected by CUR,DDP and their combination.Results: Compared with either Curcumin or cisplatin alone,combining curcumin at 10,15 or 20 μmol/L with cisplafin at l or 2 mg/L respectively increased the growth inhibition rate of A54 cells(all P<0.05) significanfly,suggesting synergistic actions of CUR and DDP can synergistically enhanced cell death in some extent.The apotosis ratio also increased according to the CUR and DDP concentration,and especially when DDP was combined with CUR.A549 cells showed G2/M stage arrest after CUR administration(P<0.01),and showed A549 cell S stage arrest with DDP administration(P<0.01).Conclusion: Both CUR and DDP could enhance the growth inhibition and apoptosis of human lung cancer A549 cells in a concentration-dependent pattern.When they were combined,the effect could be enhanced in a synergistic or additive pattern.