Graves病甲状腺内树突细胞与体液免疫紊乱的关系

目的　探讨Graves病(GD)甲状腺内树突细胞 (DC)与其体液免疫紊乱的关系。方法用抗S 100蛋白抗体SP免疫组化法对 34例GD和 5例正常对照甲状腺内的DC进行定位和半定量研究。用表达重组促甲状腺激素 (TSH)受体的人胚肾细胞检测术前血清中的甲状腺刺激性抗体(TSAb)。对甲状腺内DC的浸润密度与血清TSAb值进行相关分析。结果　正常甲状腺内未见DC,在所有被检GD患者的甲状腺内均可见到DC异常增多,且大多数DC与其周围甲状腺上皮细胞或间质浸润淋巴细胞密切接触。GD患者甲状腺内DC的浸润密度与血清TSAb值密切正相关 (r=0 4461,P<0 01)。结论　GD患者甲状腺内异常增多的DC与其体液免疫紊乱有密切关系,在其自身免疫反应的发生发展中起着重要作用。

The relationship between intrathyroidal dendritic cells and humoral immune disorder in Graves' disease

OBJECTIVE: To investigate the relationship between intrathyroidal dendritic cells and humoral immune disorder in Graves' disease. METHODS: With the use of S-100 protein antibodies and the help of SP immunohistochemical method, the number and distribution of S-100 protein positive dendritic cells were observed in thyroid glands from 34 patients with Graves' disease and 5 controls. Serum thyroid stimulating antibodies (TSAb) before operation were measured with human embryo-kidney cells expressing the recombinant thyrotrophin receptor. The correlation between the infiltrating degree of intrathyroidal dendritic cells and the values of serum TSAb was analyzed in patients with Graves' disease. RESULTS: In normal thyroid glands, no S-100 protein positive dendritic cells were found. Dendritic cells were seen in all observed thyroid glands from patients with Graves' disease. Most of the dendritic cells were seen in close contact with the adjacent thyroid epithelial cells or infiltrating lymphocytes. The infiltrating degree of intrathyroidal dendritic cells in Graves' disease correlated closely with the values of serum TSAb (r = 0.4461, P < 0.01). CONCLUSION: It is suggested that the intrathyroidal dendritic cells have a close relation with humoral immune disorder and play an important role in the initiation and/or maintenance of thyroid autoimmune reaction in Graves' disease.