全反式维甲酸对阿糖胞苷诱导HL-60细胞凋亡的影响

目的 :探讨 HL- 6 0细胞经全反式维甲酸 (ATRA)作用后对化疗药物阿糖胞苷 (Ara- C)诱导凋亡敏感性的变化。方法 :应用光镜检查凋亡细胞形态 ,DNA电泳检查梯状条带及流式细胞仪检测细胞周期分布、凋亡细胞率和 bcl- 2蛋白表达的阳性细胞率和相对荧光强度 (MFI)。结果 :ATRA0 .3mg/ L 作用 HL- 6 0细胞 72 h后 ,S期细胞显著减少至 32 .9% (P<0 .0 5 ) ,G0 / G1 期细胞明显增加达 5 8.5 % (P<0 .0 5 ) ,bcl- 2阳性细胞率和 MFI分别下降至 18%和 0 .6 3(P<0 .0 5 ) ;Ara- C1.5 m g/ L 作用 HL- 6 0细胞 4h,凋亡细胞率为 5 5 .1% ,DNA电泳见明显的梯状条带。当 HL- 6 0细胞经 ATRA0 .3m g/ L 作用 72 h后再加 Ara- C1.5 m g/ L 继续培养 4h,细胞凋亡率明显减少至 34 .4% (P<0 .0 5 ) ,DNA电泳见梯状条带亮度减弱。结论 :ATRA降低 HL- 6 0细胞对 Ara- C诱导凋亡敏感性 ,其机制可能与 ATRA阻滞 G0 / G1 期细胞进入 S期有关

THE EFFECT OF ATRA ON THE APOPTOSIS IN HL 60 CELLS INDUCED BY Ara C

Objective:To investigate the effect of ATRA on the apoptosis in HL 60 cells induced by Ara C.Methods:By means of light microscopy,DNA electrophoresis and flow cytometric analysis to detect apoptotic cells,DNA ladders and the percentage of apoptotic cells and the cells in every stage,percentage expression and the mean fluorescence intensity(MFI) of bcl 2,respectively.Results:After the cells were incubated with ATRA 0.3 mg/L for 72 hours,the percentage of cells in S stage obviously reduced to 32.9% ( P <0.05),the percentage of cells in G 0/G 1 stage greatly increased up to 58.5% ( P <0.05),respectively.After the cells were treated with Ara C 1.5 mg/L for 4 hours,the percentage of apoptotic cells was 55.1%.After the cells were incubated with ATRA 0.3 mg/L for 72 hours and subsequently treated with Ara C 1.5mg/L for 4 hours,the percentage of apoptotic cells obviously reduced to 34.4%( P <0.05).DNA electrophoresis of ATRA in combination with Ara C treated cells showed less bright DNA ladder than Ara C treated cells.Conclusion:ATRA offers HL 60 cells resistance to apoptosis induced by Ara C,whose mechanism may be related to an increase in amount of cells in G 0/G 1 stage after the cells incubated with ATRA.