一株放线菌发酵液的抗菌活性及稳定性研究 |
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引用本文: | 康颖倩,金方,刘涛华,牟丽丽,吕倩,王颜颜,曾佳,胡晓霞. 一株放线菌发酵液的抗菌活性及稳定性研究[J]. 贵阳医学院学报, 2014, 39(5): 625-629 |
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作者姓名: | 康颖倩 金方 刘涛华 牟丽丽 吕倩 王颜颜 曾佳 胡晓霞 |
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作者单位: | 1. 贵阳医学院微生物学教研室,贵州贵阳,550004 2. 贵阳医学院生物化学与分子生物学教研室,贵州贵阳,550004 3. 贵阳医学院生理教研室,贵州贵阳,550004 |
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基金项目: | 国家自然基金地方项目,贵州省社会发展科技攻关项目,贵州省卫生厅科技项目,贵阳市科技局社会发展与民生计划 |
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摘 要: | 目的:研究放线菌菌株GYM39-2发酵液对临床常见病原性细菌及真菌的抗菌活性,同时观察温度、光照、pH值及紫外线等理化因素对发酵液稳定性的影响.方法:利用纸片扩散法测定放线菌株GYM39-2发酵液对临床病原菌的抗菌谱,观察改变温度、光照、pH及紫外线等理化因素时对发酵液的抗菌活性及稳定性的影响.结果:菌株GYM39-2对金黄色葡萄球菌、大肠埃希菌、乙型副伤寒沙门菌、白念珠菌及新生隐球菌均有抗菌活性,发酵液对温度、光照的改变及紫外线的照射均有较好的稳定性,pH在5~7之间发酵液抗菌活性最强.结论:菌株GYM39-2发酵液具有良好的抗菌活性及稳定性,能为相关研究提供菌株资源.
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关 键 词: | 放线菌 发酵液 微生物敏感性试验 抗菌活性 稳定性 |
A Study on Antibiotic Activity and Stability of Fermentation Broth of A Strain of Actinomycetes |
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Affiliation: | KANG Yingqian,JIN Fang,LIU Taohua,MOU Lili,LV Qian,WANG Yanyan,ZENG Jia( 1.Department of Microbiology, Guiyang Medical College, Guiyang 550004 Guizhou, China; 2.Department of Biochemistry and Molecular Biology, Guiyang Medical College, Guiyang 550004, Guizhou, China;) |
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Abstract: | Objective:To study the antibacterial and anti-fungal activities of the fermentation broth from an actinomyocetes strain,GYM39-2,and to verify the stability of the broth under different biochemical and physical conditions.Methods:Tests on bacterial and fungal susceptibility to the broth were carried out with Kirby-Bauey method,and the the broth stability under different temperatures,illuminations,pH,and ultraviolet conditions were observed.Results:The broth showed good antibacterial and anti-fungal ability against Staphylococcus aureus,Escherichia coli,Salmnella paratyphi B,Candida albicans and Cryptococcus neoformans.The changes of temperature,illumination and ultraviolet did not affect the brothg stability and the optimal pH range was 5 ~7.Conclusion:The fermentation broth of the actinomyocetes strain,GYM39-2 has good antibacterial and anti-fungal ability.The results of this study provides new strain resource for novel antibiotics,enzymes and other biologically active substances,for both basic research and commercial application. |
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Keywords: | actinomyocetes fermentation broth microbial sensitivity test antibacterial activity stability |
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