不同配比辛芍组方对PC12细胞氧糖剥夺损伤的保护作用

目的：观察不同配比辛芍组方对PC12细胞氧糖剥夺损伤的保护作用,探讨组方的最佳配比.方法：采用连二亚硫酸钠（Na2S2O4）建立PC12细胞氧糖剥夺损伤（oxygen glucose deprivation,OGD）模型,将细胞分为空白组、模型组、阳性组（尼莫地平）、辛芍药物不同配比组（灯盏细辛与赤芍质量比分别为5∶0、4∶1、3∶2、2∶3、1∶4、0∶5）,检测细胞存活率、乳酸脱氢酶（LDH）释放量、超氧化物歧化酶（SOD）活性、丙二醛（MDA）和一氧化氮（N0）含量.结果：与正常组比较,模型组的细胞存活率和SOD活性显著降低（P＜0.01）,细胞LDH释放量、MDA和NO水平显著升高（P＜0.01）;与模型组比较,辛芍2∶3配比组显著提升细胞存活率（P＜0.05）,同时辛芍3∶2和2∶3配比组均显著降低细胞LDH释放量以及MDA和NO水平（P＜0.05）,提高SOD活性（P＜0.05）.结论：辛芍2∶3配比对PC12细胞氧糖剥夺损伤的保护作用较好.

Protective Effects of Different Proportions of Herba erigerontis and Radix paeoniae rubra on PC12 Cells against Oxygen Glucose Deprivation Induced Injury

Objective： To investigate the optimum proportion for the formula of Herba erigerontis and Radix paeoniae rubra through comparative investigations of the protective effects on PC12 cell against oxygen glucose deprivation （OGD）-induced injury. Methods： The OGD-induced injury model was es- tablished by adopting Na2S204. Cells were divided into control group, OGD group, positive group （ni- modipine）, formula group （ proportion as 5： 0,4： 1,3 ： 2,2： 3,1 ： 4,0： 5 ）. It was aimed to detect cell survival, lactate dehydrogenase （LDH） release, superoxide dismutase （SOD） activity, malondialde- hyde （MDA） and Nitric Oxide （NO）. Results： Compared with control group, the OGD model de- creased the survival rate and the activity of SOD （P 〈0.01 ）, and increased the LDH release, the lev- el of MDA and NO（P 〈0.01 ） ; Compared with OGD group, the 2：3 formula group significantly in- creased the survival rate （ P 〈 0.05 ）, while the 3 ： 2 and 2 ： 3 formula groups were significantly de- creased the LDH release, the level of MDA and NO （ P 〈 0.05 ）, increased SOD activity （ P 〈 0.05 ）. Conclusions： The 2：3 formula of Herba erigerontis and Radix paeoniae rubra has significant protective effects on the PC12 cells injured by OGD.