| 荭草提取物中总黄酮含量测定方法研究 |
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| 引用本文： | 陈思颖,谭丹,朱迪,郑林,兰燕宇. 荭草提取物中总黄酮含量测定方法研究[J]. 贵阳医学院学报, 2014, 39(4): 463-465 |
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| 作者姓名： | 陈思颖 谭丹 朱迪 郑林 兰燕宇 |
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| 作者单位： | 1. 贵阳医学院贵州省药物制剂重点实验室,贵州贵阳,550004
2. 贵阳医学院民族药与中药开发应用教育部工程研究中心,贵州贵阳,550004 |
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| 基金项目： | 国家自然科学基金项目，贵州省中药现代化科技产业研究开发专项 |
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| 摘 要： | 目的：建立荭草提取物中总黄酮含量测定方法.方法：采用紫外分光光度法,以荭草素为对照品,对荭草提取物中总黄酮进行含量测定.结果：建立紫外分光光度法测定荭草提取物中的总黄酮,荭草素对照品浓度在0.003 3～0.053 0 g/L范围内与吸光度线性关系良好,A=0.023 33C-0.000 61,r =0.999 9;平均回收率为101.3％,RSD为2.0％.结论：建立的分析方法操作简便、准确可靠,可作为荭草提取物的质量检测方法.
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| 关 键 词： | 荭草 黄酮类 分光光度法,紫外线 |
Study on the Method for Determination of Total Flavonoids in Polygonum orientale Extract |
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| CHEN Siying,TAN Dan,ZHU Di,ZHENG Lin,LAN Yanyu. Study on the Method for Determination of Total Flavonoids in Polygonum orientale Extract[J]. Journal of Guiyang Medical College, 2014, 39(4): 463-465 |
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| Authors: | CHEN Siying TAN Dan ZHU Di ZHENG Lin LAN Yanyu |
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| Affiliation: | CHEN Siying, TAN Dan , ZHU Di, ZHENG Lin , LAN Yanyu ( 1. Guizhou Provincial Key Laboratory of Pharmaceutics, Guiyang Medical College, Guiyang 550004, Guizhou, China ; 2. Engineering Research Center of Ministry Education for the Development and Application of Ethnic Medicine and TCM, Guiyang Medical College, Guiyang 550004, Guizhou, China) |
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| Abstract: | Objective ： To establish the method for determination of total flavonoids in Polygonum ori- entale extract. Methods： The total flavonoid contents in Polygonum orientale extract were determined by using UV spectrophotometry with orientin as a standard product. Result： The method for determina- tion of total flavonoids in Polygonum orientale extract by using UV spectrophotometry was established. Orientin and the absorbanee has a good linear relationship in the range of 0. 003 3 - 0.053 0 g/L, r = 0. 999 9. The average recovery rate was 101.3%, the RSD was 2.0%. Conclusion： The method was simple, accurate and reliable, and can be used to determine the quality of Polygonum orientale extract. |
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| Keywords: | Polygonum orientale flavones spectrophotometry,ultraviolet |
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