HPLC法分析左旋多巴及其有关物质

目的：建立高效液相色谱法分析左旋多巴的含量及其有关物质，并对有关物质进行定位。方法：采用Agilent TC-C18色谱柱（150mm×4．6mm，5μm）；以0．1％三氟乙酸溶液-乙腈（96：4）为流动相；流速1．0ml·min^-1；检测波长为280nm；柱温：室温。结果：左旋多巴和L-酪氨酸分别在0．06～0．61mg·ml^-1和5．01～50．12μg·ml^-1范围内线形关系良好，r=0．9999，各杂质均能达到很好的分离。结论：该方法能有效的用于左旋多巴的定量及有关物质的检测。

Determination of Levodopa and its Related Substances by HPLC

Objective： To establish a method for determination of levodopa and its related substances by HPLC. Method： The chromatographic column of Agilent TC - C18 （ 150 mm × 4.6 mm,5 μm ）was used , the mobile phase was 0.1% trifluoroacetic acid - acetonitrile （96：4 ） with flow rate of 1.0 ml · min ^- 1, the detection wavelength was set at 280 nm and the chromatography was operated at room temperature. Result： The calibration curve of levodopa and L -tyrosine was in good linearity over the range of 0.06 -0.61 mg · ml^-1 and 5.01 - 50.12 μg · ml^-1 （ r = 0. 999 9 ） , respectively. Related substances could be separated well. Conclusion： This method is convenient and accurate for the assay of levodopa and also suitable to identify of its related substances.