采用多指标成分评价全天麻胶囊品质的研究

 目的 完善全天麻胶囊的质量控制方法，建立多指标成分评价全天麻胶囊质量的方法。方法 采用HPLC方法；Phenomenex Hydro-RP 80A(4.6 mm×150 mm，4 μm )色谱柱；流动相为乙腈-0.1%磷酸，梯度洗脱：流速1 mL·min^-1；柱温：40 ℃；检测波长：270 nm。结果 天麻素、对羟基苯甲醇、对羟基苯甲醛和巴利森苷进样量分别在0.40～6.4 μg(r=0.999 7)， 0.064～1.0 μg(r=0.999 8)， 0.020～0.32 μg(r =0.999 7)， 0.60～9.5 μg(r=0.999 9)与峰面积呈良好线性关系，平均回收率(n=9)分别为100.8%（RSD 2.3%）、100.5%（RSD 3.4%）、101.9%（RSD 2.1%）、100.4%（RSD 2.5%）；25批次样品中被测成分总量具有较好的一致性（RSD 3.4%）。结论 该方法可用于评价全天麻胶囊的质量。

Quality Evaluation of Tall Gastrodia Capsules by Simultaneous Determination of the Contents of Multi-Constituents

OBJECTIVE To establish a method for simultaneous determination of the contents of muilti-constituents in tall gastrodia capsules for quality control.METHODS The HPLC was performed on a Phenomenex Hydro-RP 80A(4.6 mm×150 mm,4 μm) column with a mixture of methanol-0.1% H3PO4 solution as the mobile phase in a linear gradient elution mode.The flow rate was 1 mL·min-1,and the column temperature was set at 40 ℃.The detection wave length was set at 270 nm.RESULTS The linear ranges were 0.40-6.4 μg(r=0.999 7) for gastrodin,0.064-1.0 μg(r =0.999 8) for 4-hydroxybenzyl alcohol,0.020-0.32μg(r =0.999 7) for 4-hydroxybenzaldehyde,0.60-9.5 μg(r=0.999 9) for parishin,respectively.The average recovery were 100.8%(RSD 2.3%)、100.5%(RSD 3.4%)、101.9%(RSD 2.1%)、100.4%(RSD 2.5%),respectively.Six constituents were determined.The average content of the 6 constituents of 25 samples were 2.189%(RSD 3.40%).CONCLUSION The method is reliable and can be used for the quality evaluation of tall gastrodia capsules.