食管癌中特异表达的人类omega蛋白(Igll3)基因的序列分析及意义

目的筛选食管癌中差异表达的基因,进一步了解食管癌发生的分子机制。方法利用荧光差异显示技术(DD-PCR)从食管癌组织相对癌旁组织中获得差异表达片段,对这些片段进行克隆和序列分析。通过在GenBank中同源性检索,查找差异片段相对应的同源基因。利用定量PCR检测验证该基因表达的差异性。设计引物获得该基因全长。结果通过DD-PCR得到3个差异片段,分别为人类10号常染色体上的开放阅读框99;人类信号转导和激活转录因子2及人类omega蛋白(Igll3)。定量PCR验证的结果表明,Igll3在食管癌组织中的表达量高于癌旁组织。设计引物得到该基因全长,测序表明该基因长为711bp,编码236aa。结论 Igll3在食管癌组织中异常表达,可能在食管癌发生过程中起着重要的作用。

Expression and Structure Analysis of Calcium Binding Protein 1(Cabin1) in Esophageal Cancer

Objective To further investigate the molecular mechanism of esophageal carcinogenesis and screen out the genes expressed differentially in esophageal cancer.Methods mRNA differential display(DD-PCR)was employed to search differentially expressed fragments,some of which were cloned and sequenced.For example:Homo sapiens chromosome 10 open reading frame 99(C10orf99),Homo sapiens signal transducer and activator of transcription 2(STAT2),Homo sapiens immunoglobulin lambda-like polypeptide 3 by homology analysis in GenBank,corresponding homologous genes of those fragments were found.The corresponding genes of those differentially expressed fragments were validated by real-time quantitative PCR.Results By DD-PCR,four differentially expressed fragments were obtained,with one corresponding to immunoglobulin lambda-like polypeptide 3(Igll3).The result of real-time quantitative PCR showed that the expression level of Igll3 in esophageal cancer tissue was significantly higher than that in normal tissue.The ORF of Igll3 was 711 base pairs long and encoded 236 amino acids.Conclusion The over-expression of Igll3 in esophageal cancer may indicate an important role in esophageal carcinogenesis.