MicroRNA-181在食管癌组织中的表达

目的：探讨 microRNA-181（miR-181）在食管癌组织及细胞系中的表达及其与食管癌临床病理参数的关系。方法 RT-PCR 检测56对食管鳞癌、癌旁正常组织及食管癌细胞系 KYSE450、EC109中 miR-181的表达，并分析其表达水平与临床病理参数及生存的关系。结果与癌旁正常组织比较，食管鳞癌组织中miR-181表达明显升高（P <0.05），食管鳞癌细胞系 KYSE450、EC109中 miR-181表达水平与正常永生化食管上皮细胞 Het-1a 中表达水平比较差异有统计学意义（P <0.05）。食管鳞癌组织中 miR-181的表达量与患者的肿瘤大小、分化程度及转移情况有关。结论 miR-181在食管癌组织及食管癌细胞系中高表达，并与肿瘤大小、分化程度及转移情况相关，提示 miR-181能够作为食管癌治疗的新靶点。

Expression of MicroRNA-181 in the Esophageal Cancer

Objective To investigate the expression of miR-181 in the esophageal cancer and cell line,and the relationship between miR-181 with the clinical significance of esophageal cancer. Methods The expression level of miR-181 was determined by RT-PCR in the 56 human esophageal cancer tissue specimens and matched tumor-adja-cent esophageal tissue specimens,and esophageal cancer cell line KYSE450,EC109. The association of miR-181 ex-pression with clinicopathological characteristics in the patients with esophageal cancer was analyzed. Results Com-pared with the tumor-adjacent esophageal specimens,miR-181 was significantly high-regulated in the esophageal cancer tissue specimens(P < 0. 05). The miR-181 expression was elevated in the esophageal cell line KYSE450, EC109 compared with the Het-1a cell line(P < 0. 05). In the esophageal cancer tissue,the miR-181 expression was related with tumor size,pathological differentiation and metastasis. Conclusion Highly-expressed miR-181 is ob-served in the esophageal cancer tissues and cell lines,and it has significant correlation with tumor size,pathological differentiation and metastasis. The miR-181 represents a new potential therapeutic target for esophageal cancer treat-ment.