GANT61对肺癌细胞H1703和A549的活力和侵袭力的影响

【目的】观察新型Hh通路抑制剂GANT61对肺癌细胞H1703和A549的生长抑制情况。【方法】培养H1703和A549细胞，加入8．00μmol／L GANT6172h后采用MTT法检测细胞生长情况；Western blot法观察GANT61对Gli-1和Gli-2蛋白表达的影响；观察GANT61对细胞侵袭能力的影响。【结果】H1703和A549细胞培养24h、48h和72h光吸收值均明显低于H1703和A549细胞空白组（P〈0．05）；H1703和A549细胞加入GANT6124h后Gli-1和Gli-2蛋白相对表达量分别为（0．12±0．01），（0．09±0．01）和（0．41±0．05），（0．15±0．01）较空白组均明显减少，且差异有显著性（P〈0．05）；H1703和A549加入GANT6124h平均穿膜细胞数分别为（44．35±8．84）个和（67．12±4．71）个，明显低于H1703和A549空白组（130．12±7．83）个和（160．21±4．55）个（P〈0．05）。【结论]GANT61能明显抑制H1703和A549的细胞活力和侵袭能力，可能成为肺癌靶向治疗的新药物。

A Study of GANT61 on Growth Inhibition of Lung Cancer Cell Liness H1703 and A549

Objective]To observe the growth inhibition of GANT61 ,a Hedgehog (Hh) pathway inhibitor ,in lung cancer cell lines H1703 and A549 .[Methods] H1703 and A549 cells were cultured .Cell growth was assessed by M TT assay after 72h exposure to 8 mol/L of GANT61 .Western blot was used to observe the impact of GANT61 on protein expression of Gli1 and Gli2 ;Effect of GANT61 on the invasion ability of cells was also evalu‐ated .[Results]In the H1703 and A549 cells exposed to GANT61 for 24h ,48h ,and 72h ,light absorption values from M TT assay were significantly lower than in the H1703 and A549 cells without treatment of GANT61 for the same time points ( P < 0 .05) .The relative expressions of Gli‐1 and Gli‐2 proteins in H1703 and A549 cells(West‐ern Blot quantification for 0 .12 ± 0 .01 and 0 .41 ± 0 .05 ,respectively) were significantly reduced (0 .09 ± 0 .01 and 0 .15 ± 0 .01 ,respectively) after 24h treatment of GANT61 ( P < 0 .05) .The average numbers of transmembrane cells after GANT61 for 24h were 44 .35 ± 8 .84 in H1703 and 67 .12 ± 4 .71 in A549 cells ,while the numbers in the H1703 and A549 with mock treatment were 130 .12 ± 7 .83 and 160 .21 ± 4 .55 ,respectively .There were significant differences ( P < 0 .05) .[Conclusion]GANT61 can significantly inhibit viability and invasion of lung cancer H1703 and A549 cells and may potentially become a new drug for targeted therapy of lung cancer .