牙痛灵喷雾剂对小鼠实验性疼痛的镇痛作用

目的：观察牙痛灵喷雾剂的镇痛作用，为其临床应用提供一定的实验依据。方法：制备小鼠实验性疼痛模型，观察牙痛灵喷雾剂的镇痛作用。（1）扭体法：取昆明种小鼠50只，随机分为5组，每组10只，雌雄各半。空白对照组灌胃给予生理盐水10mL／kg；牙痛灵喷雾剂低、中、高剂量组分别灌胃牙痛灵喷雾剂0．36、1．08、3．24g／kg。阳性对照组灌胃阿司匹林0．4g／kg。每日用药1次，10d后观察各组动物腹腔注射醋酸后5～20min内的扭体反应次数。（2）热板法：将小鼠放在（55±1）℃的热板上，以小鼠舔后足为疼痛反应指标，观察小鼠的热痛阈值。选用痛觉阈值在5～30s的雌性小鼠50只，随机分为5组，每组10只，分组与给药方法与（1）同。给药10d后记录各组小鼠热板法痛阂值，比较各组间的差异。结果：（1）扭体法实验结果显示，牙痛灵喷雾剂0．36、1．08、3．24g／kg灌胃10d使腹腔注射醋酸所致的小鼠扭体反应次数较空白对照组明显减少，对扭体反应次数的抑制率分别为25．6％、30．6％和43．5％（P〈0．05，P〈0．01）。（2）热板法实验结果显示，给药前各组小鼠热痛阈值无统计学差异（P〉0．05）；给药10d后牙痛灵喷雾剂各剂量组小鼠热痛阂值均较给药前显著提高，与空白对照组比较，牙痛灵喷雾剂组给药后热痛阈值显著延长（P〈0．05，P〈0．01）。结论：牙痛灵喷雾剂对小鼠实验性疼痛具有良好的镇痛作用。

Analgesic effect of Yatongling Spray on experimental pain in mice

AIM: To investigate the analgesic effect of Yatongling Spray and to provide an experimental basis for its clinical use. METHODS: Experimental pain models of mice were established to investigate the analgesic effect of Yatongling Spray. (1) Writhing test: Fifty Kunming mice were divided into 5 groups ( n = 10 in each group, 5 male and 5 female) . The mice were gavaged with physiological saline 10 mL/kg in control group, with Yatongling Spray 0.36, 1.08, 3.24 g/kg in Yatongling Spray groups, and with aspirin 0.4 g/kg in aspirin group ( positive control group ) . Drugs were given once a day for 10 d. After the last administration , the writhing response times of mice were recorded during 5-20 min after intraperitoneal injection of acetic acid. (2)Hot plate test: A hot plate with a temperature of (55 ± 1) ℃ was used to test the pain threshold of mice. The time from putting on the hot plate to licking postpedes was defined as pain threshold time of mice. Fifty female mice with pain threshold times from 5 to 30 s were divided into 5 groups as described above. The pain threshold time was recorded after drug administration for 10 d. RESULTS: (1 ) Compared with control group, the writhing response times of mice were significantly decreased with inhibition rates of 25.6 % , 30.6 % and 43.5 % after gavaged with Yatongling Spray 0.36, 1.08 and 3.24 g/kg for 10 d( P < 0.05, P<0.01), respectively. (2)The pain thresholds were not significantly different among the groups before drug administration ( P > 0.05 ) . After gavaged with Yatongling Spray 0.36, 1.08 and 3.24 g/kg for 10 d, the pain thresholds were significantly prolonged compared with that of control group ( P < 0.05, P < 0.01). CONCLUSION: Yatongling Spray has an analgesic effect on experimental pain of mice.