胎盘组织荧光PCR诊断早期先天梅毒的研究

目的评价胎盘组织荧光PCR(VQ—PCR)在先天梅毒早期诊断中的作用。方法对34例梅毒孕妇和30例非梅毒对照孕妇的胎盘和脐带组织进行梅毒螺旋体DNA的荧光定量PCR检测，并与常规临床及血清学综合诊断方法比较。结果34例梅毒孕妇的胎盘组织中，11例荧光PCR为阳性，阳性率为32．35％，30例非梅毒孕妇的胎盘和脐带样本荧光PCR均为阴性；以常规临床及血清学综合诊断方法为标准，则荧光PCR与常规方法结果的灵敏度为82％，特异性100％，约登指数0．82。显示在诊断先天梅毒新生儿时，FQ—PCR至少有不低于常规综合诊断方法的价值。结论与常规临床血清学综合诊断方法相比，荧光PCR均有明显的优势。

Study on detection of placental Treponema pallidum DNA for diagnosis of congenital syphilis by using FQ-PCR.

Objective To evaluate the role of FQ-PCR in detection of placental Treponema pallidum DNA for diagnosis of congenital syphilis. Methods Thirty-four placental specimens from pregnant women with syphilis and thirty specimens from health pregnant women were blindly evaluated with: (1) FQ-PCR for placental Treponema pallidum DNA;(2) Traditional comprehensive approach for diagnosis of congenital syphilis. The results were compared with each other. Results Among the 34 placentas from syphilitic women who completed pregnancy, 11 had positive FQ-PCR. None of the 30 infants from health mother had positive result. FQ-PCR for placental Treponema pallidum DNA showed comparable sensitivity superior to the traditional comprehensive approach for diagnosis of congenital syphilis. Conclusion FQ-PCR for placental Treponema pallidum DNA could be an addition to conventional diagnostic tools.