大孔树脂纯化无花果叶总黄酮

目的:研究大孔树脂纯化无花果叶总黄酮的工艺条件.方法:采用静态吸附法对5种大孔树脂进行筛选,动态吸附法优选大孔树脂纯化无花果叶总黄酮工艺.结果:无花果叶总黄酮的纯化工艺为上样浓度2 g·L-1,上样速率2 BV·h-1,上样体积3 BV,2 BV去离子水冲洗；4 BV 80％乙醇以2 BV·h-1流速洗脱,收集洗脱液；此工艺条件下无花果叶总黄酮的转移率为77.6％,样品产物中总黄酮质量分数51.3％.结论:D140型大孔树脂对无花果叶总黄酮具有良好的富集纯化效果.

Purification of Total Flavonoids from Leaves of Ficus carica by Macroporous Resin

Objective: To study on purification technology conditions of total flavonoids from leaves of Ficus carica by macroporous resin. Method: Five models of macroporous resins were selected by static adsorption,purification technology of total flavonoids from leaves of F. carica with macroporous resin was optimized by dynamic adsorption. Result: Optimum purification technology was as follows:concentration of sample 2 g·L^-1,velocity of sample 2 BV·h^-1, volume of sample 3 BV,eluted with 2 BV deionized water and abandoned eluate, then eluted with 4BV 80% ethanol at speed of 2 BV·h^-1,eluate was collected;Yield of total flavonoids was 51.3% at this technology conditions, and recovery rate of total flavonoids was 77.6%. Conclusion: D140 macroporous resin could purify total flavonoids from leaves of F. carica effectively.