| 响应面法优化黄芩中黄芩苷闪式提取工艺 |
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| 引用本文： | 范成杰,江道峰,凌宗士. 响应面法优化黄芩中黄芩苷闪式提取工艺[J]. 中国实验方剂学杂志, 2012, 18(6): 48-51 |
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| 作者姓名： | 范成杰 江道峰 凌宗士 |
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| 作者单位： | 成都中医药大学药学院,中药材标准化教育部重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,成都611137 |
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| 摘 要： | 目的:利用响应面分析方法优化黄芩苷闪式提取工艺条件.方法:以HPLC测定黄芩苷含量,以黄芩苷得率为指标,单因素试验考察乙醇体积分数、提取时间及料液比；采用Box-Behnken设计、响应面分析统计学方法对工艺参数进行优化.结果:最佳工艺条件为14倍量70％乙醇提取102 s,在此条件下得到黄芩苷的实测值达13.588％,理论值可达13.592％,两者较吻合.结论:闪式提取法是一种高效、快速提取黄芩中黄芩苷的方法.
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| 关 键 词： | 黄芩苷 闪式提取 响应面法 |
| 收稿时间： | 2011-07-02 |
Optimization of Flash-type Extraction Technology of Baicalin from Scutellaria baicalensis by Response Surface Methodology |
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| FAN Cheng-jie,JIANG Dao-feng and LING Zong-shi. Optimization of Flash-type Extraction Technology of Baicalin from Scutellaria baicalensis by Response Surface Methodology[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(6): 48-51 |
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| Authors: | FAN Cheng-jie JIANG Dao-feng LING Zong-shi |
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| Affiliation: | School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese herbal medicines, Ministry of Education, Laboratory of Resources System Research and Development Utilization of Traditional Chinese Medicine Constructioned by China and Sichuan Province, Chengdu 611137, China;School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese herbal medicines, Ministry of Education, Laboratory of Resources System Research and Development Utilization of Traditional Chinese Medicine Constructioned by China and Sichuan Province, Chengdu 611137, China;School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Key Laboratory of Standardization of Chinese herbal medicines, Ministry of Education, Laboratory of Resources System Research and Development Utilization of Traditional Chinese Medicine Constructioned by China and Sichuan Province, Chengdu 611137, China |
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| Abstract: | Objective: To optimize flash-type extraction technology of baicalin from Scutellaria baicalensis by response surface methodology (RSM). Method: With yield of baicalin as index, the content of baicalin was determined by HPLC. Concentration of ethanol,extracting time and liquid-material ratio were selected by single-factor test;Technology parameters were optimized by Box-Benhnken design and response surface analysis statistical method. Result: Optimal extraction technology conditions were as follows:concentration of ethanol 73%,extraction time 102 s, liquid-solid ratio 14: 1 (mL: g),under these optimum conditions,measured value of baicalin was 13.588%,it was in line with theoretical value of 13.592%. Conclusion: Flash-type extraction was proved to be a kind of efficient and rapid method for extraction of baicalin from S. baicalensis. |
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| Keywords: | baicalin flash-type extraction response surface methodology |
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