3,5-二硝基水杨酸法联合苯酚-浓硫酸法测定不同产地黄精中多糖含量

目的:建立一种操作简便,可排除黄精药材本身所含单糖干扰的多糖含量测定方法,建立合理客观地评价黄精药材的质量.方法:联合3,5-二硝基水杨酸法和苯酚-浓硫酸法,通过紫外分光光度法分别测定黄精原药材中还原糖和总糖的含量,取两者之差即为药材中多糖的含量,并从重复性、精密度、稳定性以及加样回收率等方面进行了方法学考察.结果:本方法具有良好的适用性,不同产地的黄精药材多糖含量测定结果表明云南产黄精药材中多糖含量较高.结论:本实验样品处理简单、方法的重复性、精密度、稳定性及加样回收率均符合要求,可用于评价黄精生药材的质量.

Determination of Polysaccharide in Rhizoma Polygonatii by Phenol-Sulfuric Acid and DNS Methods

Objective: To establish a simple determination method of polysaccharide in Rhizoma Polygonatii, which is not influenced by the content of monosaccharide. Method: The total sugar was determined by phenol-sulfuric acid method while the concentration of reducing sugar was determined by 3,5-dinitrosalicylic acid (DNS) method, the contentration of polysaccharide=C_t(the concentration of total sugar)-C_s(the concentration of reducing sugar). Result: It was used to evaluate the content of polysaccharide collected in different area, the concentrations of polysaccharide in Polygonatum Rhizoma collected fromYunnan province are relatively higher. Conclusion: The results showed above methods are convenient, stable and accurate,which can be used for quality evaluation of Polygonatum Rhizoma and its preparations.