HPLC测定蛇胆川贝液中的防腐剂

目的:建立蛇胆川贝液中6种防腐剂的含量测定方法,评价当前该品种防腐剂的使用情况.方法:采用ODSHypersil C18反相高效液相色谱柱(4.6 mm ×250 mm,5μm),以甲醇-0.025 mol-L -磷酸二氢钠为流动相梯度洗脱,检测波长苯甲酸为228 nm,山梨酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯为258 nm.结果:6种防腐剂完全分离,回收率为99.2％ ～102.0％.结论:本方法准确易行,可用于蛇胆川贝液的质量控制.

Determination of Preservatives in Shedan Chuanbei Mixture by HPLC

Objective:To bring forward a method of determining 6 preservatives in Shedan chuanbei mixture and figure out the state of quality of preservative in this mixture.Method:A ODS Hypersil C18(4.6mm × 250 mm,5 μm) column was used with menthol-0.025 mol.L-1 sodium phosphate monobasic as the mobile phase under condition of gradient elution.The detection wavelength of benzoic acid was at 228 nm and the detection wavelength of sorbic acid and parahydroxybenzoate esters were at 258 nm.Result:All the 6 preservatives were completely separated,the average recovery was in the range of 99.2%-102.04%.Conclusion:The method can be used to control the quality of Shedan chuanbei mixture,which provides the advantage of quickness,simplicity and repeatability.