尖叶假龙胆中norswertianolin的含量测定

目的:利用反相高效液相色谱法对尖叶假龙胆中norswertianolin成分进行含量测定。方法:采用Shim-pack VP-ODS-C18柱(4.6 mm×150 mm,5μm),流动相0.1%磷酸水溶液(A)-乙腈(B)(80:20),流速1.0 mL.min-1,柱温25℃,紫外检测波长254 nm。结果:尖叶假龙胆中norswertianolin在9.6～192 mg.L-1线性关系良好(r=0.999 5);仪器精密度RSD0.67%(n=6);方法重复性RSD 1.88%(n=6);样品在12 h内稳定;平均回收率为99.8%(RSD 1.31%)。结论:本法快速、准确、重复性好,可用于蒙药尖叶假龙胆中norswertianolin成分的含量测定。

Determination of the Norswertianolin in Gentianella acuta

Objective:To determine norswertianolin of Gentianella acuta by reverse-phase HPLC method.Method:The determination of norswertianolin was performed on a shim-pack VP-ODS-C18 column(4.6 mm ×150 mm,5 μm).The mobil phase was 0.1% phosphoric acid solution(A)-acetonitrile(B)(80:20).The flow rate was 1.0 mL·min-1.Ultraviolet absorption detector was set at 254 nm and column temperature at 25 ℃.Norswertianolin of G.acuta was separated well.Result:The result indicated that the linear range of norswertianolin of G.acuta was between 9.6-192 mg·L-1(r=0.999 5);the relative standard deviations of instrument precision was 0.67%(n=6);the relative standard deviations of method reproducibility was 1.88%(n=6);the relative standard deviations for stability was 1.68%;the average recovery was 99.8%(n=6).Conclusion:The results indicated that this developed assay method was rapid,accurate,and reliable and could be readily used as a quality control method for the norswertianolin.