| 基于近红外光谱的白芍药材快速分析 |
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| 引用本文： | 李家春,萧伟,孙兰,夏翠英,王振中.基于近红外光谱的白芍药材快速分析[J].中国实验方剂学杂志,2012,18(6):57-60. |
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| 作者姓名： | 李家春 萧伟 孙兰 夏翠英 王振中 |
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| 作者单位： | 江苏康缘药业股份有限公司,江苏连云港222001;中药制药过程新技术国家重点实验室,江苏连云港222001 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09504004) |
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| 摘 要： | 目的:采用近红外(NIR)光谱技术建立一种快速测定白芍药材芍药苷的方法.方法:运用HPLC测定样品中芍药苷的含量,运用偏最小二乘(PLS)法建立含量如NIR光谱之间的多元校正模型,对未知样品进行含量预测.结果:校正集的内部交差验证均方差RMSECV为0.019 7,校正模型中真实值与预测值之间的相关系数(R2)为0.969 6,外部验证均方差(RMSEP)为0.046 1.结论:近红外漫反射光谱法快速、简便、无损,可用于白芍药材中芍药苷含量的快速检测,对其他药材中指标性成分的测定也有一定的参考价值.
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| 关 键 词： | 近红外光谱法 白芍 快速分析 |
| 收稿时间： | 2011/5/13 0:00:00 |
Rapid Analysis of Paeoniae Radix Alba by Near Infrared Spectroscopy |
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| LI Jia-chun,XIAO Wei,SUN Lan,XIA Cui-ying and WANG Zhen-zhong.Rapid Analysis of Paeoniae Radix Alba by Near Infrared Spectroscopy[J].China Journal of Experimental Traditional Medical Formulae,2012,18(6):57-60. |
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| Authors: | LI Jia-chun XIAO Wei SUN Lan XIA Cui-ying and WANG Zhen-zhong |
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| Institution: | Jiangsu Kanion Pharmaceutical Co.Ltd, Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharnacontical Prde, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.Ltd, Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharnacontical Prde, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.Ltd, Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharnacontical Prde, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.Ltd, Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharnacontical Prde, Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co.Ltd, Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharnacontical Prde, Lianyungang 222001, China |
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| Abstract: | Objective: To develop a rapid NIR method for determination of paeoniflorin in paeony root. Method: HPLC was used as a reference method to determine the content of paeoniflorin. Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method. Result: the RMSECV of the model for paeoniflorin was 0.019 7,the correlation coefficients of the calibration models was 0.969 7,the RMSEP was 0.046 1. Conclusion: The NIR method was rapid,simple,and noninvasive and that is applied to determine the content of paeoniflorin in paeony root. It can offer reference to content determination of other Chinese herbs. |
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| Keywords: | near-infrared spectroscopy Paeoniae Radix Alba rapid analysis |
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