蒽酮-硫酸比色法测定香菇多糖含量

目的：建立一种香菇多糖原料药中香菇多糖含量的测定方法。方法：以右旋糖苷为对照品,使用蒽酮-硫酸比色法测定。结果：在右旋糖苷浓度为10.004-50.020ug/ml范围内,625nm处浓度与吸光度线性关系良好,A=0.0183C-0.0019,R=0.9997（n=5）,重复性RSD=0.51%（n=6）,回收率平均值98.69%,RSD=0.50%（n=6）,与以香菇多糖精制品为对照品的测定值对比,结果良好。结论：本法简便、可行、准确,适用于香菇多糖原料药中香菇多糖含量的测定。

Determination of the Lentinan Content by Anthrone-sulfuric Acid Colorimetry

Objective：To establish a method for determination of the Lentinan content in Lentinan API.Method：Determine by anthrone-sulfuric acid colorimetry,taking Dextran as the reference control.Result：In the Dextrane concentration within 10.004-50.020ug/ml,concentration and absorbance showed positive linear relations at 625nm,A=0.0183C-0.0019,R=0.9997（n=5）,with the repeatability RSD=0.51%（n=6）,average recovery rate reach to 98.69%,RSD=0.50%（n=6）.Compared with the result by taking purified Lentinan as the reference control,the result is acceptable.Conclusion：This method is simple,feasible and accurate,therefore it can be used to determine the Lentinan content in Lentinan API.