| 膜联蛋白V真核表达载体的构建 |
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| 引用本文： | 黄瑞滨,洪梅,仲任,宋善俊. 膜联蛋白V真核表达载体的构建[J]. 广西医科大学学报, 2004, 21(2): 171-173 |
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| 作者姓名： | 黄瑞滨 洪梅 仲任 宋善俊 |
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| 作者单位： | 华中科技大学同济医学院附属协和医院血液病学研究所,武汉,430022;华中科技大学同济医学院附属协和医院血液病学研究所,武汉,430022;华中科技大学同济医学院附属协和医院血液病学研究所,武汉,430022;华中科技大学同济医学院附属协和医院血液病学研究所,武汉,430022 |
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| 基金项目： | 国家自然科学基金 ( No:3 0 2 0 0 10 9) |
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| 摘 要： | 目的：构建人膜联蛋白V基因真核表达载体，探讨人膜联蛋白V的生理功能。方法：PCR方法扩增含EcoRI及BamHI酶切位点的膜联蛋白V基因序列，对真核表达载体pcDNA3．1(-)和膜联蛋白V基因扩增产物进行双酶切，用连接酶将二者连接并转化到大肠杆菌BL21，重组质粒经测序鉴定，称为pcDNA3．1(-)AxV。结果：酶切琼脂糖电泳分析pcDNA3．1(-)AxV中含有膜联蛋白V基因，序列分析表明与文献报道的序列完全一致。结论：成功地构建了人膜联蛋白V基因的真核表达载体，为研究其生理作用奠定了基础。
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| 关 键 词： | 膜联蛋白V 基因 真核表达载体 |
| 修稿时间： | 2004-01-10 |
CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR CONTAINING HUMAN ANNEXIN V GENE |
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| Huang Ruibin,Hong Mei,Zhong Ren,et al.. CONSTRUCTION OF EUKARYOTIC EXPRESSION VECTOR CONTAINING HUMAN ANNEXIN V GENE[J]. Journal of Guangxi Medical University, 2004, 21(2): 171-173 |
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| Authors: | Huang Ruibin Hong Mei Zhong Ren et al. |
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| Abstract: | Objective:To construct eukaryotic expression plasmid of human annexin V gene for further study.Methods:Annexin V gene containing EcoRI and BamHI endoenzyme site was obtained by using PCR.Double enzyme digestion was conducted for pcDNA3 1(-) and PCR product of annexin V gene.Both fragements were connected by using ligase and transferred to BL21.Reconstituted plasmid was examined by auto sequenceing assay and was namedas pcDNA3.1(-)AxV.Result:The pcDNA3 1(-)AxV plasmid was confirmed to contain correct nucleotide sequence for human annexin V gene by restriction endonuclease digestion and sequence analysis.Conclusion:A eukaryotic expression plasmid containing human annexin V gene was successfully constructed and was helpful for the research for the function of annexin V. |
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| Keywords: | annexin V gene eukaryotic expression plasmid |
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