改构体酸性成纤维细胞生长因子对肠缺血再灌注损伤中AP-1表达的影响

目的：观察外源性给予改构体酸性成纤维细胞生长因子（aFGF）后,肠缺血-再灌注损伤中转录因子AP-1（fos/jun复合物）表达规律。方法：以大鼠肠系膜上动脉（SMA）夹闭造成肠缺血-再灌注损伤模型,并将动物随机分为假手术组（C）、生理盐水对照组（R）、改构体治疗组（F）。除假手术组外,其余各组动物均于缺血45 min后2、6、12、24h活杀,取小肠组织标本,免疫组化检测原癌基因c-fos、c-jun表达规律。结果：在正常大鼠,原癌基因c-fos、c-jun分布在小肠绒毛上皮细胞的肠腔侧、侧壁和小肠隐窝朝向隐窝腔的一侧的细胞膜上。缺血和再灌注的初期,原癌基因c-fos、c-jun的表达未发生明显变化,随着再灌注时间的延长逐渐增强。改构体aFGF使原癌基因c-fos、c-jun的表达有明显的增强。缺血和再灌注使PCNA的表达增加,在再灌注后6 h达到高峰。F组PCNA的表达较R组相应时间点显著增加（P〈0.05）。结论：转录因子AP-1与PCNA表达一致,在缺血-再灌注损伤的修复中起积极作用;改构体aFGF对此有促进作用。

INFLUENCES OF AFGF ON AP-1 EXPRESSION IN RATGUT ISCHEMIA-REPERFUSION INJURY MODEL

Objective：To investigate the influences of exogenous acidic fibroblast growth factor（aFGF） on the AP-1（fos/jun complex） expression in rat intestine ischemia-reperfusion injury model.Methods：Rat gut I-R injury models were established by clamping the superior mesenteric artery（SMA）,and the animals were divided randomly into sham-operation group（C）,saline control group（R） and aFGF treatment group（F）.The expression level of c-fos、c-jun and PCNA were measured with immuno-histology and semi-quantification with MIG2000 combined graphic analytic system at 2,6,12 and 24h after clamping and reperfusion.Results： The expression of c-fos、c-jun remained unchanged at the early stages of the injury but increased gradually with the prolonging of reperfusion in the control group;while the expression level of c-fos、c-jun and PCNA was significantly increased and reached its peak at 6h in the treatment group（P〈0.05）.Conclusions：The expression of AP-1 is closely related to that of PCNA and plays a positive role in the I-R injury repair process which is enhanced by aFGF.