关节游离体软骨细胞生物学特性的初步观察

目的观察关节游离体软骨细胞的生物学特性，寻找组织工程软骨种子细胞来源。方法分别取游离体软骨标本5例，正常软骨标本2例和骨关节炎软骨标本6例，组织学观察其细胞分布、尿嘧啶脱氧核苷末端转移酶介导的三磷酸脱氧核苷缺口标记法观察细胞凋亡，细胞分离培养观察原代软骨细胞形态，并进行比较。结果与正常软骨、骨关节炎软骨比较，游离体软骨细胞分布密度较高，细胞凋亡明显．体外培养时原代细胞已呈成纤维细胞样形态。结论游离体软骨细胞已具有成纤维细胞特性，不宜直接作为组织工程的种子细胞来源。

PRELIMINARY OBSERVATION OF BIOLOGICAL CHARACTERS OF CHONDROCYTES IN ARTICULAR LOOSE BODY

OBJECTIVE: To observe the biological characters of chondrocytes in articular loose body and to find out seeding cells for cartilage tissue engineering. METHODS: Samples from 5 loose body cartilages, 2 normal articular cartilages and 6 osteoarthritis articular cartilages were collected. Part of each sample's cartilage was histologically studied to observe the chondrocytes distribution the morphologic changes by toluidine-blue staining, chondrocytes' apoptosis by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate-biotin nick end-labeling (TUNEL). The rest of each cartilage was digested and isolated by 0.25% trypsin and 0. 2% collagenase II, and then were cultivated in 10%DMEM. Their morphologic changes were observed 24h later. Comparison was made btween three cartilages. RESULTS: Compared with normal cartilage and osteoarthritis articular cartilage, the cells density was higher, their lacunars were larger, cells distribution was irregular, and apoptosis was more apparent in loose body cartilage. CONCLUSION: The characters of chondrocytes from loose body is more like fibroblasts so they can not serve as seeding cells directly for cartilage tissue engineering.