化学发光微粒子免疫分析法筛查梅毒螺旋体特异性抗体的临床研究

目的：用化学发光微粒子免疫分析法（CM IA ）联合梅毒螺旋体明胶凝集试验（T PPA ）和甲苯胺红不加热血清试验（TRUST）检测梅毒抗体（TP-Ab），探讨CMIA在筛查梅毒螺旋体特异性抗体中的临床应用价值。方法采用CMIA对住院和门诊患者进行TP-Ab筛查，阳性标本再进行TPPA和TRUST 的检测，对比分析3种检测方法的检验结果的符合率。结果25030例血清标本经CM IA筛查共检出T P-Ab阳性732例，检出率为2．9％。以TPPA和TRUST对阳性标本进行联合检测的阳性检出率分别为78．7％、37．2％。以 TPPA为标准参考方法， CM IA S/CO在1～＜9的阳性符合率为29．7％～90．1％，S/CO≥9的阳性符合率为100．0％。结论 CM IA可取代传统的非密螺旋体抗原血清试验用于高通量梅毒抗体的筛查。

Research of the clinical application value of chemiluminescent microparticle immunoassay in screening reponema palli -dum specific antibody

Objective To explore the clinical application value of chemiluminescent microparticle immunoassay （CMIA）in screening treponema pallidum specific antibody by using CMIA ,treponena pallidum particle agglutination experiment （TPPA） and syphilis toluidine red untreated serum test （TRUST） to detect reponema pallidum antibody （TP-Ab） .Methods Used CMIA method to screen TP-Ab for inpatient and outpatient ,positive specimens were test-ed by TPPA and TRUST ,compared and analyzed the coincidence rate of the test result of the 3 detection methods . Results 25 030 serum samples were detected TP-Ab by CMIA ,732 were positive ,detection rate was 2 .9% .Positive samples were detected by TPPA and TRUST ,the positive detection rates were 78 .7% and 37 .2% respectively .With TPPA as standard reference method ,positive coincidence rates of S/CO in 1 to 9 by CMIA method were 29 .7% to 90 .1% ,S/CO in 9 or more was 100 .0% .Conclusion CMIA method could replace the traditional non treponema antigen in serum test for high throughput screening of TP-Ab .