瑞芬太尼对家兔缺血脑组织水通道蛋白-4表达的影响

目的观察瑞芬太尼对家兔缺血再灌注损伤脑组织水通道蛋白-4（AQP-4）表达的影响。方法健康家兔25只，随机分为假手术组、缺血模型组、瑞芬太尼组。以双侧颈总动脉阻断＋控制性低血压法建立家兔全脑缺血损伤模型，再灌注1h，6h，24h末，测定脑组织含水量，观察AQP4的表达，再灌注24h末，观察脑组织超微结构变化。结果与假手术组比较，模型组家兔脑组织含水量及脑组织内AQP4表达在1h，6h和24h逐渐升高（P〈0．05）；与模型组比较，瑞芬太尼组脑组织含水量与AQP-4表达明显降低（P〈0．05）。结论瑞芬太尼可能通过降低脑组织内AQP-4表达，减轻脑水肿，保护脑缺血损伤。

The Effect of Remifentanil on the Expression of AQP-4 in Rabbits Brain Induced by Cerebral Ischemia-Reperfusion Injury

Objective To study the protective and resuscitative effects of remifentanil on the expression of AQP-4 in rabbits brain. Methods Twenty-five healthy rabbits were randomly divided into three groups ： the sham operation group,the cerebral ischemia group and the remifentanil group. The cerebral isehemia-reperfusion injury model was established by blocking bilateral carotid artery plus the method of controlled hypotension. The moisture capacity of the brain tissue and the expression of AQP-4 was observed at the end of 1 h,6h and 24h after reperfusion. The change of ul- trastructural organization was observed at the end of 24h after reperfusion. Results Compared with sham operation group,the moisture capacity of the brain tissue and the expression of AQP-4 in the cerebral ischemia group were increased gradually after 1 h,6h and 24h（P 〈0.05 ） ;Compared with the cerebral ischemia group, the brain water content and AQP-4 expression in the remifentanil group were obviously lower（ P 〈 0. 05 ）. Conclusion Remifentanil may have cerebral protective effect through downregulating AQP-4 expression in cerebral ischemie area and reducing brain edema.